Universität zu Köln

Higgins, Paul G. ORCID: 0000-0001-8677-9454, Schneiders, Thamarai ORCID: 0000-0002-7758-7925, Hamprecht, Axel ORCID: 0000-0003-1449-5780 and Seifert, Harald (2010). In Vivo Selection of a Missense Mutation in adeR and Conversion of the Novel bla(OXA-164) Gene into bla(OXA-58) in Carbapenem-Resistant Acinetobacter baumannii Isolates from a Hospitalized Patient. Antimicrob. Agents Chemother., 54 (12). S. 5021 - 5028. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 0066-4804

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Abstract

The mechanism of stepwise acquired multidrug resistance in Acinetobacter baumannii isolates from a hospitalized patient was investigated. Thirteen consecutive multidrug-resistant isolates were recovered from the same patient over a 2-month period. The Vitek 2 system identified the isolates as meropenem-sensitive Acinetobacter lwoffii; however, molecular identification showed that the isolates were A. baumannii. Etest revealed that the isolates were meropenem resistant. The presence of oxacillinase (OXA)-type enzymes were investigated by sequencing. The clonal relatedness of isolates was assessed by pulsed-field gel electrophoresis (PFGE). Expression of the genes encoding the efflux pumps AdeB and AdeJ was performed by semiquantitative real-time reverse transcription-PCR (qRT-PCR). The adeRS two-component system was sequenced. All isolates had identical PFGE fingerprints, suggesting clonal identity. The first six isolates were positive for the novel bla(OXA-164) gene. The following seven isolates, recovered after treatment with a combination of meropenem, amikacin, ciprofloxacin, and co-trimoxazole showed an increase of >7-fold in adeB mRNA transcripts and a missense mutation in bla(OXA-164), converting it to bla(OXA-58). Sequencing revealed a novel mutation in adeR. These data illustrate how A. baumannii can adapt during antimicrobial therapy, leading to increased antimicrobial resistance.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Schneiders, Thamarai UNSPECIFIED orcid.org/0000-0002-7758-7925 UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491091
DOI:	10.1128/AAC.00598-10
Journal or Publication Title:	Antimicrob. Agents Chemother.
Volume:	54
Number:	12
Page Range:	S. 5021 - 5028
Date:	2010
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	0066-4804
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EFFLUX PUMP; ESCHERICHIA-COLI; RECEIVER DOMAIN; EXPRESSION; SEQUENCE; ADEABC; PCR; SUSCEPTIBILITY; CALCOACETICUS; TIGECYCLINE Multiple languages Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49109