Universität zu Köln

Liu, Shangxi, Blumbach, Katrin, Eastwood, Mark, Denton, Christopher P., Eckes, Beate, Krieg, Thomas, Abraham, David J. and Leask, Andrew (2010). Expression of integrin beta 1 by fibroblasts is required for tissue repair in vivo. J. Cell Sci., 123 (21). S. 3674 - 3683. CAMBRIDGE: COMPANY OF BIOLOGISTS LTD. ISSN 1477-9137

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Abstract

In tissue repair, fibroblasts migrate into the wound to produce and remodel extracellular matrix (ECM). Integrins are believed to be crucial for tissue repair, but their tissue-specific role in this process is poorly understood. Here, we show that mice containing a fibroblast-specific deletion of integrin beta 1 exhibit delayed cutaneous wound closure and less granulation tissue formation, including reduced production of new ECM and reduced expression of alpha-smooth muscle actin (alpha-SMA). Integrin-beta 1-deficient fibroblasts showed reduced expression of type I collagen and connective tissue growth factor, and failed to differentiate into myofibroblasts as a result of reduced alpha-SMA stress fiber formation. Loss of integrin beta 1 in adult fibroblasts reduced their ability to adhere to, to spread on and to contract ECM. Within stressed collagen matrices, integrin-beta 1-deficient fibroblasts showed reduced activation of latent TGF beta. Addition of active TGF beta alleviated the phenotype of integrin-beta 1-deficient mice. Thus integrin beta 1 is essential for normal wound healing, where it acts, at least in part, through a TGF beta-dependent mechanism in vivo.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Liu, Shangxi UNSPECIFIED UNSPECIFIED UNSPECIFIED Blumbach, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Eastwood, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Denton, Christopher P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eckes, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED Krieg, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Abraham, David J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leask, Andrew UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-493501
DOI:	10.1242/jcs.070672
Journal or Publication Title:	J. Cell Sci.
Volume:	123
Number:	21
Page Range:	S. 3674 - 3683
Date:	2010
Publisher:	COMPANY OF BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX; GROWTH-FACTORS; KERATINOCYTE MIGRATION; GENE-EXPRESSION; FOCAL ADHESIONS; WOUND REPAIR; SKIN; MYOFIBROBLAST; CONTRACTION; MICE Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49350