Kohler, Maxie, Thomas, Andreas ORCID: 0000-0003-1199-0743, Walpurgis, Katja, Terlouw, Koen, Schaenzer, Wilhelm and Thevis, Mario (2010). Detection of His-tagged Long-R-3-IGF-I in a black market product. Growth Horm. IGF Res., 20 (5). S. 386 - 391. EDINBURGH: CHURCHILL LIVINGSTONE. ISSN 1096-6374

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Abstract

Objective: Performance-enhancing substances are illicitly used in elite or amateur sports and may be obtained from the black market due to a cheaper and easier availability. Although various studies have shown that black market products frequently do not contain the declared substances, enormous amounts of illegally produced and/or imported drugs are confiscated from athletes or at customs with alarming results concerning the outcome of the analyses of the ingredients. This case report describes the identification of His-tagged Long-R-3-IGF-I, which is usually produced for biochemical studies, in an injection vial. Design: The ingredients were isolated by immunoaffinity purification and identified by nano-UPLC, high-resolution/high accuracy mass spectrometry of the intact and trypsinated substance and by an enzyme-linked immunosorbent assay. Results: (Tandem) mass spectra characterized the protein as Long-R-3-IGF-I with a His(6)-tag attached to the C-terminus by the linker amino acids Leu-Glu. Conclusion: His-tags are commonly added to proteins during synthesis to allow a convenient and complete purification of the final product and His-tags are subsequently removed by specific enzymes when being attached to the N-terminus. The effects of His-tagged Long-R-3-IGF-I in humans have not been elucidated or described and the product may rather be a by-product from biochemical studies than synthesized for injection purposes. (C) 2010 Growth Hormone Research Society. Published by Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kohler, MaxieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thomas, AndreasUNSPECIFIEDorcid.org/0000-0003-1199-0743UNSPECIFIED
Walpurgis, KatjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Terlouw, KoenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaenzer, WilhelmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-495613
DOI: 10.1016/j.ghir.2010.07.001
Journal or Publication Title: Growth Horm. IGF Res.
Volume: 20
Number: 5
Page Range: S. 386 - 391
Date: 2010
Publisher: CHURCHILL LIVINGSTONE
Place of Publication: EDINBURGH
ISSN: 1096-6374
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GROWTH-FACTOR-I; MASS-SPECTROMETRIC DETERMINATION; IGF-I; TISSUE DISTRIBUTION; PLASMA-CLEARANCE; AFFINITY TAGS; HORMONE; INSULIN; PURIFICATION; PERFORMANCEMultiple languages
Cell Biology; Endocrinology & MetabolismMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/49561

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