Oestreicher, Iris, Almeida, Jorge Reis, Campean, Valentina, Rauh, Manfred ORCID: 0000-0002-4852-7157, Plank, Christian, Amann, Kerstin and Doetsch, Joerg (2010). Changes in 11 beta-hydroxysteroid dehydrogenase type 2 expression in a low-protein rat model of intrauterine growth restriction. Nephrol. Dial. Transplant., 25 (10). S. 3195 - 3204. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2385

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Abstract

Methods. IUGR rats after maternal low-protein diet (n = 17) were compared with controls (n = 18). At 70 and 120 days of age, in situ distribution of 11 beta-HSD2 gene and protein expression was investigated by RT-PCR of microdissected tubules and immunohistochemistry. For in situ localization studies, double staining for 11 beta-HSD2 and calbindin was used. Serum levels of corticosterone and dehydrocorticosterone were measured by tandem mass spectrometry. Results. In IUGR rats, intra-arterial blood pressure significantly increased at Day 120 of life. Serum corticosterone/dehydrocorticosterone ratios and 11 beta-HSD2 mRNA in total kidney were not altered in IUGR animals. However, 11 beta-HSD2 mRNA concentration was significantly lower in microdissected tubuli of IUGR animals (Day 120: 0.18 +/- 0.14 vs 1.00 +/- 0.32 rel. units in controls; P < 0.05). In IUGR animals, immunostaining scores for 11 beta-HSD2 were significantly lower than in controls (P < 0.05). Double staining with calbindin showed lower expression of 11 beta-HSD2 in distal segments of the distal tubule. Conclusions. Our data indicate lower gene and protein expression of the pre-receptor enzyme 11 beta-HSD2 in IUGR animals when looking at specific renal compartments, but not in total kidney extracts. Thus, lower 11 beta-HSD2 as a mechanism for hypertension later in life might be missed without methods for in situ detection.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Oestreicher, IrisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Almeida, Jorge ReisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Campean, ValentinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rauh, ManfredUNSPECIFIEDorcid.org/0000-0002-4852-7157UNSPECIFIED
Plank, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Amann, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doetsch, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-496068
DOI: 10.1093/ndt/gfq354
Journal or Publication Title: Nephrol. Dial. Transplant.
Volume: 25
Number: 10
Page Range: S. 3195 - 3204
Date: 2010
Publisher: OXFORD UNIV PRESS
Place of Publication: OXFORD
ISSN: 1460-2385
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BLOOD-PRESSURE; NEPHRON NUMBER; GLUCOCORTICOID ACTION; GENE-EXPRESSION; HYPERTENSION; PREGNANCY; CORTISOL; RECEPTOR; DISEASEMultiple languages
Transplantation; Urology & NephrologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/49606

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