Universität zu Köln

Solbach, Marcel Dominik ORCID: 0000-0001-7073-7428, Bonkowski, Michael and Dumack, Kenneth ORCID: 0000-0001-8798-0483 (2021). Novel Endosymbionts in Rhizarian Amoebae Imply Universal Infection of Unrelated Free-Living Amoebae by Legionellales. Front. Cell. Infect. Microbiol., 11. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988

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Abstract

Legionellales-infected water is a frequent cause of local outbreaks of Legionnaires' disease and Pontiac fever. Decontaminations are difficult because Legionellales reproduce in eukaryotic microorganisms (protists). Most often, Legionellales have been isolated from amoebae; however, the culture-based sampling methods are taxonomically biased. Sequencing studies show that amoebae in the cercozoan class Thecofilosea are dominant in soils and wastewater treatment plants, prompting us to screen their capability to serve as potential hosts of endosymbiotic bacteria. Environmental isolates of Thecofilosea contained a surprising richness of endosymbiotic Legionellales, including Legionella. Considering the widespread dispersal of Legionellales in apparently unrelated amoeboid protist taxa, it appears that the morphotype and not the evolutionary origin of amoebae determines their suitability as hosts for Legionellales. We further provide a protocol for gnotobiotic cultivation of Legionellales and their respective hosts, facilitating future genomic and transcriptomic research of host-symbiont relationships.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Solbach, Marcel Dominik UNSPECIFIED orcid.org/0000-0001-7073-7428 UNSPECIFIED Bonkowski, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Dumack, Kenneth UNSPECIFIED orcid.org/0000-0001-8798-0483 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-561451
DOI:	10.3389/fcimb.2021.642216
Journal or Publication Title:	Front. Cell. Infect. Microbiol.
Volume:	11
Date:	2021
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	2235-2988
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Immunology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56145