Ndjenda Ii, Magloire Kanyou, Nguelefack-Mbuyo, Elvine Pami ORCID: 0000-0002-5754-7686, Atsamo, Albert Donatien, Fofie, Christian Kuete, Fodem, Chamberlin, Nguemo, Filomain ORCID: 0000-0002-2236-6797 and Nguelefack, Telesphore Benoit ORCID: 0000-0002-5006-6927 (2021). Antihypertensive Effects of the Methanol Extract and the Ethyl Acetate Fraction from Crinum zeylanicum (Amaryllidaceae) Leaves in L-NAME-Treated Rat. Evid.-based Complement Altern. Med., 2021. LONDON: HINDAWI LTD. ISSN 1741-4288

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Arterial hypertension (AHT) is a leading cardiovascular disease, with a high negative impact on the quality of life. Crinum zeylanicum (C. zeylanicum) leaves extract is used in the West region of Cameroon to treat AHT and heart problems. This study aimed to investigate the antihypertensive effect of C. zeylanicum extract in N-omega-nitro-L-arginine methyl ester- (L-NAME-) induced hypertensive rats. The aqueous extract of C. zeylanicum (LAE) was obtained by lyophilizing the juice of triturated fresh leaves. The methanol extract (LME) prepared by maceration of the dried leaves was further partitioned to chloroform (LCF), ethyl acetate (LEAF), and residual (LRF) fractions. The total polyphenol, flavonoid content, and antiradical potentials of these extracts were determined. The curative antihypertensive and renal function protective effects of LME and LEAF were evaluated in vivo on L-NAME-induced hypertensive rats. Hypertension was induced in rats by oral administration of L-NAME (30 mg/kg/day) for 3 consecutive weeks. Thereafter, plant extracts were administered orally at the doses of 30, 60, and 120 mg/kg/day, concomitantly with L-NAME for three other weeks. Body weight, heart rate, and arterial blood pressure were measured at the end of each week throughout the experimental period. At the end of the treatment, 24-hour urine and plasma were collected to assay nitric oxide (NO), creatinine, and protein. The results revealed that LEAF has the higher content of total polyphenol and flavonoid and exhibited the best antiradical potential. Moreover, treatment of hypertensive rats with LME and LEAF significantly (p<0.001) reduced AHT and heart rate. LME and LEAF significantly increased rat's body mass, plasmatic NO, and urinary creatinine and reduced urine NO and protein contents as compared to the L-NAME group. LME and its LEAF possess potent antihypertensive effects and further protect the renal function in L-NAME-induced hypertensive rats, thus supporting the use of C. zeylanicum in the management of AHT.

Item Type: Journal Article
CreatorsEmailORCIDORCID Put Code
Nguelefack-Mbuyo, Elvine PamiUNSPECIFIEDorcid.org/0000-0002-5754-7686UNSPECIFIED
Nguemo, FilomainUNSPECIFIEDorcid.org/0000-0002-2236-6797UNSPECIFIED
Nguelefack, Telesphore BenoitUNSPECIFIEDorcid.org/0000-0002-5006-6927UNSPECIFIED
URN: urn:nbn:de:hbz:38-575664
DOI: 10.1155/2021/2656249
Journal or Publication Title: Evid.-based Complement Altern. Med.
Volume: 2021
Date: 2021
Publisher: HINDAWI LTD
Place of Publication: LONDON
ISSN: 1741-4288
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
Integrative & Complementary MedicineMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57566


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