Koeditz, Barbara, Stog, Andreas, Goebel, Heike, Heidegger, Isabell, Fries, Jochen, Heidenreich, Axel and Von Brandenstein, Melanie (2021). Vimentin 3 Expression in Prostate Cancer Cells. Anticancer Res., 41 (1). S. 169 - 175. ATHENS: INT INST ANTICANCER RESEARCH. ISSN 1791-7530

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Abstract

Background/Aim: Vimentin3 (Vim3) was recently described as a tumour marker for the direct discrimination between benign and malignant kidney tumours. Here, we examined its expression in prostate cancer (PCa) cell lines and the regulation of its expression by endothelin receptors. Materials and Methods: Prostate cancer cell lines (PC3, DU145, LNCap) were incubated with endothelin 1 (ET-1), BQ123 [endothelin A receptor (ETAR) antagonist], BQ788 [endothelin B receptor (ETBR) antagonist), BQ123+ET-1, BQ788+ET-1 for 24 h and a scratch assay was performed. Cell extracts were analysed by western blotting and qRT-PCR. Results: ET-1 induced Vim3 overexpression. Blocking the ETBR in the different prostate cancer cell lines yielded a higher migration rate, whereby Vim3 expression was significantly increased. Conclusion: Vim3 concentration increases in cell lines without a functional ETBR and may be used as a marker for PCas where ETBR is frequently methylated.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Koeditz, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stog, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Goebel, HeikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heidegger, IsabellUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fries, JochenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heidenreich, AxelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Von Brandenstein, MelanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-575723
DOI: 10.21873/anticanres.14762
Journal or Publication Title: Anticancer Res.
Volume: 41
Number: 1
Page Range: S. 169 - 175
Date: 2021
Publisher: INT INST ANTICANCER RESEARCH
Place of Publication: ATHENS
ISSN: 1791-7530
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ENDOTHELIN-1; METASTASIS; RECEPTORMultiple languages
OncologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57572

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