Universität zu Köln

Schuetz, Markus B., Renner, Alexander M. ORCID: 0000-0002-8267-6690, Ilyas, Shaista, Le, Khan, Guliyev, Mehrab, Krapf, Philipp, Neumaier, Bernd ORCID: 0000-0001-5425-3116 and Mathur, Sanjay (2021). F-18-Labeled magnetic nanovectors for bimodal cellular imaging. Biomater. Sci., 9 (13). S. 4717 - 4728. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 2047-4849

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Abstract

Surface modification of nanocarriers enables selective attachment to specific molecular targets within a complex biological environment. Besides the enhanced uptake due to specific interactions, the surface ligands can be utilized for radiolabeling applications for bimodal imaging ensured by positron emission topography (PET) and magnetic resonance imaging (MRI) functions in one source. Herein, we describe the surface functionalization of magnetite (Fe3O4) with folic acid as a target vector. Additionally, the magnetic nanocarriers were conjugated with appropriate ligands for subsequent copper-catalyzed azide-alkyne cycloaddition or carbodiimide coupling reactions to successfully achieve radiolabeling with the PET-emitter F-18. The phase composition (XRD) and size analysis (TEM) confirmed the formation of Fe3O4 nanoparticles (6.82 nm +/- 0.52 nm). The quantification of various surface functionalities was performed by Fourier-transform infrared spectroscopy (FT-IR) and ultraviolet-visible microscopy (UV-Vis). An innovative magnetic-HPLC method was developed in this work for the determination of the radiochemical yield of the F-18-labeled NPs. The as-prepared Fe3O4 particles demonstrated high radiochemical yields and showed high cellular uptake in a folate receptor overexpressing MCF-7 cell line, validating bimodal imaging chemical design and a magnetic HPLC system. This novel approach, combining folic acid-capped Fe3O4 nanocarriers as a targeting vector with F-18 labeling, is promising to apply this probe for bimodal PET/MR-studies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schuetz, Markus B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Renner, Alexander M. UNSPECIFIED orcid.org/0000-0002-8267-6690 UNSPECIFIED Ilyas, Shaista UNSPECIFIED UNSPECIFIED UNSPECIFIED Le, Khan UNSPECIFIED UNSPECIFIED UNSPECIFIED Guliyev, Mehrab UNSPECIFIED UNSPECIFIED UNSPECIFIED Krapf, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumaier, Bernd UNSPECIFIED orcid.org/0000-0001-5425-3116 UNSPECIFIED Mathur, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-576929
DOI:	10.1039/d1bm00616a
Journal or Publication Title:	Biomater. Sci.
Volume:	9
Number:	13
Page Range:	S. 4717 - 4728
Date:	2021
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	2047-4849
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IRON-OXIDE NANOPARTICLES; CONTRAST AGENTS; COMPUTED-TOMOGRAPHY; TOXICITY; PATIENT; CELLS; PROBE Multiple languages Materials Science, Biomaterials Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57692