Universität zu Köln

Wegner, Joanna, Weidenthaler-Barth, Beate, Engelbert, Julia, Knothe, Max, Braun, Claudia, Helbig, Doris, Sacher, Christopher, Kreft, Andreas, Wagner, Eva M., Ziemer, Mirjana, Meyer, Ralf G. and von Stebut, Esther (2021). Immunohistochemical markers for histopathological diagnosis and differentiation of acute cutaneous graft-versus-host disease. Exp. Dermatol., 30 (12). S. 1814 - 1820. HOBOKEN: WILEY. ISSN 1600-0625

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Abstract

Graft-versus-host disease (GvHD) is a major complication following stem-cell or solid-organ transplantation. Accurate diagnosis of cutaneous GvHD is challenging, given that drug eruptions and viral rashes may present with similar clinical/histological manifestations. Specific markers are not available. We performed the histological examination of biopsy samples from acute GvHD (aGvHD; n = 54), Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN; n = 27), maculopapular drug eruption (MDE; n = 26) and healthy controls (n = 26). Samples of aGvHD showed a decrease in Langerhans cells (LC, p = 0.0001) and an increase in macrophages (M phi, p = 0.0001) compared to healthy skin. Compared to SJS/TEN, MDE and healthy skin, aGvHD biopsies contained greater numbers of CD4(+) and CD8(+) T cells. The majority of CD4(+) T-helper cells were localized in the upper dermis, whereas cytotoxic CD8(+) T cells were found in the epidermis. Increased numbers of CD56(+) natural killer (NK) cells in the upper dermis of aGvHD skin (p = 0.007) were not observed in controls or SJS/TEN and MDE. There were no differences in elafin staining between aGvHD and the latter two conditions. Acute GvHD appears to have a distinct inflammatory cell profile (T cells/NK cells) that may aid establishing in a more accurate diagnosis, especially when used to rule out differential diagnoses such as SJS/TEN or MDE.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wegner, Joanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Weidenthaler-Barth, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED Engelbert, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Knothe, Max UNSPECIFIED UNSPECIFIED UNSPECIFIED Braun, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Helbig, Doris UNSPECIFIED UNSPECIFIED UNSPECIFIED Sacher, Christopher UNSPECIFIED UNSPECIFIED UNSPECIFIED Kreft, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Eva M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ziemer, Mirjana UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Ralf G. UNSPECIFIED UNSPECIFIED UNSPECIFIED von Stebut, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-577974
DOI:	10.1111/exd.14416
Journal or Publication Title:	Exp. Dermatol.
Volume:	30
Number:	12
Page Range:	S. 1814 - 1820
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-0625
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONSENSUS DEVELOPMENT PROJECT; CLINICAL-TRIALS; NK CELLS; SKIN; CRITERIA; EXPRESSION; DEATH Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57797