Kochan, David Z., Mawer, Julia S. P., Massen, Jennifer ORCID: 0000-0001-9649-1542, Tishinov, Kiril ORCID: 0000-0003-3211-1824, Parekh, Swati ORCID: 0000-0002-4826-1651, Graef, Martin, Spang, Anne ORCID: 0000-0002-2387-6203 and Tessarz, Peter ORCID: 0000-0002-6953-9835 (2021). The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcription. J. Cell Sci., 134 (15). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9137

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Abstract

Gene expression involves regulation of chromatin structure and transcription, as well as processing of the transcribed mRNA. While there are feedback mechanisms, it is not clear whether these include crosstalk between chromatin architecture and mRNA decay. To address this, we performed a genome-wide genetic screen using a Saccharomyces cerevisiae strain harbouring the H3K56A mutation, which is known to perturb chromatin structure and nascent transcription. We identified Puf5 (also known as Mpt5) as essential in an H3K56A background. Depletion of Puf5 in this background leads to downregulation of Puf5 targets. We suggest that Puf5 plays a role in post-transcriptional buffering of mRNAs, and support this by transcriptional shutoff experiments in which Puf5 mRNA targets are degraded slower in H3K56A cells compared to wild-type cells. Finally, we show that post-transcriptional buffering of Puf5 targets is widespread and does not occur only in an H3K56A mutant, but also in an H3K4R background, which leads to a global increase in nascent transcription. Our data suggest that Puf5 determines the fate of its mRNA targets in a context-dependent manner acting as an mRNA surveillance hub balancing deregulated nascent transcription to maintain physiological mRNA levels.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kochan, David Z.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mawer, Julia S. P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Massen, JenniferUNSPECIFIEDorcid.org/0000-0001-9649-1542UNSPECIFIED
Tishinov, KirilUNSPECIFIEDorcid.org/0000-0003-3211-1824UNSPECIFIED
Parekh, SwatiUNSPECIFIEDorcid.org/0000-0002-4826-1651UNSPECIFIED
Graef, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Spang, AnneUNSPECIFIEDorcid.org/0000-0002-2387-6203UNSPECIFIED
Tessarz, PeterUNSPECIFIEDorcid.org/0000-0002-6953-9835UNSPECIFIED
URN: urn:nbn:de:hbz:38-579127
DOI: 10.1242/jcs.259051
Journal or Publication Title: J. Cell Sci.
Volume: 134
Number: 15
Date: 2021
Publisher: COMPANY BIOLOGISTS LTD
Place of Publication: CAMBRIDGE
ISSN: 1477-9137
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
NUCLEOSOME STRUCTURE; GENE-EXPRESSION; YEAST GENES; ACETYLATION; DEGRADATION; VERSATILE; DYNAMICS; STRATEGY; PACKAGE; SET1Multiple languages
Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/57912

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