Kalka, Katharina ORCID: 0000-0002-1697-1843, Keldenich, Zoe, Carstens, Henning, Walter, Bjorn, Rauen, Ursula, Ruhparwar, Arjang, Weymann, Alexander, Kamler, Markus, Reiner, Gerald and Koch, Achim ORCID: 0000-0002-1616-4873 (2022). Custodiol-MP for ex vivo lung perfusion - A comparison in a porcine model of donation after circulatory determination of death. Int. J. Artif. Organs, 45 (2). S. 162 - 174. LONDON: SAGE PUBLICATIONS LTD. ISSN 1724-6040

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Abstract

Introduction: Ex vivo lung perfusion (EVLP) is an established technique to evaluate and eventually recondition lungs prior to transplantation. Custodiol-MP (C-MP) solution is a new solution, designed for clinical machine perfusion, that has been used for kidneys. The aim of this study was to compare the effects of EVLP with Custodiol-MP on lung functional outcomes to the gold standard of EVLP with Steen Solution (TM). Material and Methods: In a porcine EVLP model of DCDD (Donation after Circulatory Determination of Death), lungs were perfused with Steen Solution (TM) (SS, n = 7) or Custodiol-MP solution supplemented with 55 g/l albumin (C-MP, n = 8). Lungs were stored cold for 4 h in low potassium dextran solution and subsequently perfused ex vivo for 4 h. During EVLP pulmonary gas exchange, activities of lactate dehydrogenase (LDH) and alkaline phosphatase (AP) as well as levels of lactate in the perfusate were recorded hourly. Results: Oxygenation capacity differed significantly between groups (averaged over 4 h: SS 274 +/- 178 mmHg; C-MP 284 +/- 151 mmHg p = 0.025). Lactate dehydrogenase activities and lactate concentrations were significantly lower in Custodiol-MP perfused lungs. In a porcine model of DCDD with 4 h of EVLP the use of modified Custodiol-MP as perfusion solution was feasible. The use of C-MP showed at least comparable lung functional outcomes to the use of Steen Solution(TM). Furthermore C-MP perfusion resulted in significantly lower lactate dehydrogenase activity and lactate levels in the perfusate and higher oxygenation capacity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kalka, KatharinaUNSPECIFIEDorcid.org/0000-0002-1697-1843UNSPECIFIED
Keldenich, ZoeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carstens, HenningUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Walter, BjornUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rauen, UrsulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ruhparwar, ArjangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weymann, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kamler, MarkusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reiner, GeraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, AchimUNSPECIFIEDorcid.org/0000-0002-1616-4873UNSPECIFIED
URN: urn:nbn:de:hbz:38-582392
DOI: 10.1177/0391398821990663
Journal or Publication Title: Int. J. Artif. Organs
Volume: 45
Number: 2
Page Range: S. 162 - 174
Date: 2022
Publisher: SAGE PUBLICATIONS LTD
Place of Publication: LONDON
ISSN: 1724-6040
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CURRENT PRESERVATION TECHNOLOGY; MACHINE PERFUSION; FUTURE-PROSPECTS; INDUCED INJURY; COLD-STORAGE; TRANSPLANTATION; MECHANISMS; CELLSMultiple languages
Engineering, Biomedical; TransplantationMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58239

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