Pradhan, Ashis Kumar, Kandasamy, Ganapathi, Chatterjee, Upasana, Bharadwaj, Anushree, Mathew, Sam J., Dohmen, R. Jurgen and Palanimurugan, R. (2021). Ribosome-associated quality control mediates degradation of the premature translation termination product Orf1p of ODC antizyme mRNA. FEBS Lett., 595 (15). S. 2015 - 2034. HOBOKEN: WILEY. ISSN 1873-3468
Full text not available from this repository.Abstract
Decoding of OAZ1 (Ornithine decarboxylase AntiZyme 1) mRNA, which harbours two open reading frames (ORF1 and ORF2) interrupted by a naturally occurring Premature Termination Codon (PTC), produces an 8 kDa truncated polypeptide termed Orf1p, unless the PTC is bypassed by +1 ribosomal frameshifting. In this study, we identified Orf1p as an endogenous ubiquitin-dependent substrate of the 26S proteasome both in yeast and mammalian cells. Surprisingly, we found that the ribosome-associated quality control factor Rqc1 and the ubiquitin ligase Ltn1 are critical for Orf1p degradation. In addition, the cytosolic protein quality control chaperone system Hsp70/Hsp90 and their corresponding co-chaperones Sse1, Fes1, Sti1 and Cpr7 are also required for Orf1p proteolysis. Our study finds that Orf1p, which is naturally synthesized as a result of a premature translation termination event, requires the coordinated role of both ribosome-associated and cytosolic protein quality control factors for its degradation.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-586642 | ||||||||||||||||||||||||||||||||
DOI: | 10.1002/1873-3468.14147 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | FEBS Lett. | ||||||||||||||||||||||||||||||||
Volume: | 595 | ||||||||||||||||||||||||||||||||
Number: | 15 | ||||||||||||||||||||||||||||||||
Page Range: | S. 2015 - 2034 | ||||||||||||||||||||||||||||||||
Date: | 2021 | ||||||||||||||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||||||
ISSN: | 1873-3468 | ||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/58664 |
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