Welcker, Daniela, Stein, Cornelia, Feitosa, Natalia Martins, Armistead, Joy, Zhang, Jin-Li, Luetke, Steffen, Kleinridders, Andre ORCID: 0000-0002-4248-6366, Bruening, Jens C., Eming, Sabine A., Sengle, Gerhard, Niehoff, Anja, Bloch, Wilhelm and Hammerschmidt, Matthias (2021). Hemicentin-1 is an essential extracellular matrix component of the dermal-epidermal and myotendinous junctions. Sci Rep, 11 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

Full text not available from this repository.

Abstract

The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that mouse Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal-epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1(-/-) mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1(-/-) mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Welcker, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stein, CorneliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Feitosa, Natalia MartinsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Armistead, JoyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, Jin-LiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Luetke, SteffenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kleinridders, AndreUNSPECIFIEDorcid.org/0000-0002-4248-6366UNSPECIFIED
Bruening, Jens C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eming, Sabine A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sengle, GerhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niehoff, AnjaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bloch, WilhelmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hammerschmidt, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-588965
DOI: 10.1038/s41598-021-96824-4
Journal or Publication Title: Sci Rep
Volume: 11
Number: 1
Date: 2021
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
FIBULIN-4; ELASTOGENESIS; PROTEINS; ROLES; LINKMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58896

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item