Universität zu Köln

Wang, Duo, Liu, Chang, Liu, Huan, Meng, Yilei, Lin, Fang, Gu, Yanqiong, Wang, Hanrui, Shang, Mengyue, Tong, Chang, Sachinidis, Agapios, Ying, Qilong, Li, Li and Peng, Luying (2021). ERG1 plays an essential role in rat cardiomyocyte fate decision by mediating AKT signaling. Stem Cells, 39 (4). S. 443 - 458. HOBOKEN: WILEY. ISSN 1549-4918

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Abstract

ERG1, a potassium ion channel, is essential for cardiac action potential repolarization phase. However, the role of ERG1 for normal development of the heart is poorly understood. Using the rat embryonic stem cells (rESCs) model, we show that ERG1 is crucial in cardiomyocyte lineage commitment via interactions with Integrin beta 1. In the mesoderm phase of rESCs, the interaction of ERG1 with Integrin beta 1 can activate the AKT pathway by recruiting and phosphorylating PI3K p85 and focal adhesion kinase (FAK) to further phosphorylate AKT. Activation of AKT pathway promotes cardiomyocyte differentiation through two different mechanisms, (a) through phosphorylation of GSK3 beta to upregulate the expression levels of beta-catenin and Gata4; (b) through promotion of nuclear translocation of nuclear factor-kappa B by phosphorylating IKK beta to inhibit cell apoptosis, which occurs due to increased Bcl2 expression. Our study provides solid evidence for a novel role of ERG1 on differentiation of rESCs into cardiomyocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Duo UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Chang UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Huan UNSPECIFIED UNSPECIFIED UNSPECIFIED Meng, Yilei UNSPECIFIED UNSPECIFIED UNSPECIFIED Lin, Fang UNSPECIFIED UNSPECIFIED UNSPECIFIED Gu, Yanqiong UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Hanrui UNSPECIFIED UNSPECIFIED UNSPECIFIED Shang, Mengyue UNSPECIFIED UNSPECIFIED UNSPECIFIED Tong, Chang UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Ying, Qilong UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Peng, Luying UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-590939
DOI:	10.1002/stem.3328
Journal or Publication Title:	Stem Cells
Volume:	39
Number:	4
Page Range:	S. 443 - 458
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1549-4918
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59093