Universität zu Köln

Graf, Juergen, Zhang, Chuanqiang ORCID: 0000-0002-2391-6754, Marguet, Stephan Lawrence, Herrmann, Tanja, Flossmann, Tom ORCID: 0000-0003-3158-5980, Hinsch, Robin, Rahmati, Vahid, Guenther, Madlen, Frahm, Christiane, Urbach, Anja ORCID: 0000-0002-9321-829X, Neves, Ricardo Melo, Witte, Otto W., Kiebel, Stefan J., Isbrandt, Dirk ORCID: 0000-0002-4720-1016, Huebner, Christian A., Holthoff, Knut and Kirmse, Knut ORCID: 0000-0002-9206-214X (2021). A limited role of NKCC1 in telencephalic glutamatergic neurons for developing hippocampal network dynamics and behavior. Proc. Natl. Acad. Sci. U. S. A., 118 (14). WASHINGTON: NATL ACAD SCIENCES. ISSN 1091-6490

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Abstract

NKCC1 is the primary transporter mediating chloride uptake in immature principal neurons, but its role in the development of in vivo network dynamics and cognitive abilities remains unknown. Here, we address the function of NKCC1 in developing mice using electrophysiological, optical, and behavioral approaches. We report that NKCC1 deletion from telencephalic glutamatergic neurons decreases in vitro excitatory actions of gamma-aminobutyric acid (GABA) and impairs neuronal synchrony in neonatal hippocampal brain slices. In vivo, it has a minor impact on correlated spontaneous activity in the hippocampus and does not affect network activity in the intact visual cortex. Moreover, long-term effects of the developmental NKCC1 deletion on synaptic maturation, network dynamics, and behavioral performance are subtle. Our data reveal a neural network function of NKCC1 in hippocampal glutamatergic neurons in vivo, but challenge the hypothesis that NKCC1 is essential for major aspects of hippocampal development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Graf, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Chuanqiang UNSPECIFIED orcid.org/0000-0002-2391-6754 UNSPECIFIED Marguet, Stephan Lawrence UNSPECIFIED UNSPECIFIED UNSPECIFIED Herrmann, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Flossmann, Tom UNSPECIFIED orcid.org/0000-0003-3158-5980 UNSPECIFIED Hinsch, Robin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahmati, Vahid UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenther, Madlen UNSPECIFIED UNSPECIFIED UNSPECIFIED Frahm, Christiane UNSPECIFIED UNSPECIFIED UNSPECIFIED Urbach, Anja UNSPECIFIED orcid.org/0000-0002-9321-829X UNSPECIFIED Neves, Ricardo Melo UNSPECIFIED UNSPECIFIED UNSPECIFIED Witte, Otto W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiebel, Stefan J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isbrandt, Dirk UNSPECIFIED orcid.org/0000-0002-4720-1016 UNSPECIFIED Huebner, Christian A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Holthoff, Knut UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirmse, Knut UNSPECIFIED orcid.org/0000-0002-9206-214X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-596807
DOI:	10.1073/pnas.2014784118
Journal or Publication Title:	Proc. Natl. Acad. Sci. U. S. A.
Volume:	118
Number:	14
Date:	2021
Publisher:	NATL ACAD SCIENCES
Place of Publication:	WASHINGTON
ISSN:	1091-6490
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAMMA-AMINOBUTYRIC-ACID; SYNAPTIC PLASTICITY; DEVELOPING NEOCORTEX; GABA ACTIONS; MOUSE MODEL; SHARP WAVES; KCC2; DEPOLARIZATION; TRANSMISSION; OSCILLATIONS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59680