Kirschner, K. Gwendolyn, Rosignoli, Serena ORCID: 0000-0002-4319-8761, Guo, Li ORCID: 0000-0003-0406-6966, Vardanega, Isaia ORCID: 0000-0002-3642-9381, Imani, Jafargholi ORCID: 0000-0001-8265-1656, Altmueller, Janine, Milner, G. Sara, Balzano, Raffaella, Nagel, A. Kerstin, Pflugfelder, Daniel, Forestan, Cristian ORCID: 0000-0002-1712-825X, Bovina, Riccardo, Koller, Robert, Stoecker, G. Tyll, Mascher, Martin, Simmonds, James, Uauy, Cristobal, Schoof, Heiko ORCID: 0000-0002-1527-3752, Tuberosa, Roberto, Salvi, Silvio and Hochholdinger, Frank (2021). ENHANCED GRAVITROPISM 2 encodes a STERILE ALPHA MOTIF-containing protein that controls root growth angle in barley and wheat. Proc. Natl. Acad. Sci. U. S. A., 118 (35). WASHINGTON: NATL ACAD SCIENCES. ISSN 1091-6490

Full text not available from this repository.

Abstract

The root growth angle defines how roots grow toward the gravity vector and is among the most important determinants of root system architecture. It controls water uptake capacity, nutrient use efficiency, stress resilience, and, as a consequence, yield of crop plants. We demonstrated that the egt2 (enhanced gravitropism 2) mutant of barley exhibits steeper root growth of seminal and lateral roots and an auxin-independent higher responsiveness to gravity compared to wild-type plants. We cloned the EGT2 gene by a combination of bulked-segregant analysis and whole genome sequencing. Subsequent validation experiments by an independent CRISPR/Cas9 mutant allele demonstrated that egt2 encodes a STERILE ALPHA MOTIF domain-containing protein. In situ hybridization experiments illustrated that EGT2 is expressed from the root cap to the elongation zone. We demonstrated the evolutionary conserved role of EGT2 in root growth angle control between barley and wheat by knocking out the EGT2 orthologs in the A and B genomes of tetraploid durum wheat. By combining laser capture microdissection with RNA sequencing, we observed that seven expansin genes were transcriptionally down-regulated in the elongation zone. This is consistent with a role of EGT2 in this region of the root where the effect of gravity sensing is executed by differential cell elongation. Our findings suggest that EGT2 is an evolutionary conserved regulator of root growth angle in barley and wheat that could be a valuable target for root-based crop improvement strategies in cereals.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kirschner, K. GwendolynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rosignoli, SerenaUNSPECIFIEDorcid.org/0000-0002-4319-8761UNSPECIFIED
Guo, LiUNSPECIFIEDorcid.org/0000-0003-0406-6966UNSPECIFIED
Vardanega, IsaiaUNSPECIFIEDorcid.org/0000-0002-3642-9381UNSPECIFIED
Imani, JafargholiUNSPECIFIEDorcid.org/0000-0001-8265-1656UNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Milner, G. SaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Balzano, RaffaellaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagel, A. KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pflugfelder, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Forestan, CristianUNSPECIFIEDorcid.org/0000-0002-1712-825XUNSPECIFIED
Bovina, RiccardoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koller, RobertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stoecker, G. TyllUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mascher, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Simmonds, JamesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Uauy, CristobalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schoof, HeikoUNSPECIFIEDorcid.org/0000-0002-1527-3752UNSPECIFIED
Tuberosa, RobertoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Salvi, SilvioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hochholdinger, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-599297
DOI: 10.1073/pnas.2101526118
Journal or Publication Title: Proc. Natl. Acad. Sci. U. S. A.
Volume: 118
Number: 35
Date: 2021
Publisher: NATL ACAD SCIENCES
Place of Publication: WASHINGTON
ISSN: 1091-6490
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SYSTEM ARCHITECTURE; GRAVITY RESPONSE; ELONGATION ZONE; AUXIN TRANSPORT; CAP CELLS; ARABIDOPSIS; IDENTIFICATION; ADAPTATION; DISCOVERY; MUTANTSMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59929

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item