Universität zu Köln

Santini, Laura, Halbritter, Florian ORCID: 0000-0003-2452-4784, Titz-Teixeira, Fabian ORCID: 0000-0002-7007-9039, Suzuki, Toru ORCID: 0000-0002-0174-0794, Asami, Maki ORCID: 0000-0003-3523-0984, Ma, Xiaoyan, Ramesmayer, Julia, Lackner, Andreas ORCID: 0000-0003-1168-7947, Warr, Nick ORCID: 0000-0003-0767-1360, Pauler, Florian, Hippenmeyer, Simon ORCID: 0000-0003-2279-1061, Laue, Ernest, Farlik, Matthias, Bock, Christoph ORCID: 0000-0001-6091-3088, Beyer, Andreas, Perry, Anthony C. F. and Leeb, Martin ORCID: 0000-0001-5114-4782 (2021). Genomic imprinting in mouse blastocysts is predominantly associated with H3K27me3. Nat. Commun., 12 (1). BERLIN: NATURE RESEARCH. ISSN 2041-1723

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Abstract

In mammalian genomes, differentially methylated regions (DMRs) and histone marks including trimethylation of histone 3 lysine 27 (H3K27me3) at imprinted genes are asymmetrically inherited to control parentally-biased gene expression. However, neither parent-of-origin-specific transcription nor imprints have been comprehensively mapped at the blastocyst stage of preimplantation development. Here, we address this by integrating transcriptomic and epigenomic approaches in mouse preimplantation embryos. We find that seventy-one genes exhibit previously unreported parent-of-origin-specific expression in blastocysts (nBiX: novel blastocyst-imprinted expressed). Uniparental expression of nBiX genes disappears soon after implantation. Micro-whole-genome bisulfite sequencing (mu WGBS) of individual uniparental blastocysts detects 859 DMRs. We further find that 16% of nBiX genes are associated with a DMR, whereas most are associated with parentally-biased H3K27me3, suggesting a role for Polycomb-mediated imprinting in blastocysts. nBiX genes are clustered: five clusters contained at least one published imprinted gene, and five clusters exclusively contained nBiX genes. These data suggest that early development undergoes a complex program of stage-specific imprinting involving different tiers of regulation. In most mammals, imprinted genes contain epigenetic marks that differ in each parental genome and control their parent-of-origin-specific expression. Here, the authors map imprinted genes in mouse preimplantation embryos and find that imprinted gene expression in blastocysts is mainly dependent on Polycomb-mediated H3K27me3-associated gene silencing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Santini, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Halbritter, Florian UNSPECIFIED orcid.org/0000-0003-2452-4784 UNSPECIFIED Titz-Teixeira, Fabian UNSPECIFIED orcid.org/0000-0002-7007-9039 UNSPECIFIED Suzuki, Toru UNSPECIFIED orcid.org/0000-0002-0174-0794 UNSPECIFIED Asami, Maki UNSPECIFIED orcid.org/0000-0003-3523-0984 UNSPECIFIED Ma, Xiaoyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Ramesmayer, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Lackner, Andreas UNSPECIFIED orcid.org/0000-0003-1168-7947 UNSPECIFIED Warr, Nick UNSPECIFIED orcid.org/0000-0003-0767-1360 UNSPECIFIED Pauler, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Hippenmeyer, Simon UNSPECIFIED orcid.org/0000-0003-2279-1061 UNSPECIFIED Laue, Ernest UNSPECIFIED UNSPECIFIED UNSPECIFIED Farlik, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Christoph UNSPECIFIED orcid.org/0000-0001-6091-3088 UNSPECIFIED Beyer, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Perry, Anthony C. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leeb, Martin UNSPECIFIED orcid.org/0000-0001-5114-4782 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-599416
DOI:	10.1038/s41467-021-23510-4
Journal or Publication Title:	Nat. Commun.
Volume:	12
Number:	1
Date:	2021
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EMBRYONIC STEM-CELLS; ELEMENTS; GENE; ESTABLISHMENT; INJECTION; U2AF1-RS1; MICE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59941