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Brands, Mathias and Doermann, Peter (2022). Two AMP-Binding Domain Proteins from Rhizophagus irregularis Involved in Import of Exogenous Fatty Acids. Mol. Plant-Microbe Interact., 35 (6). S. 464 - 477. ST PAUL: AMER PHYTOPATHOLOGICAL SOC. ISSN 1943-7706

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Abstract

Arbuscular mycorrhizal fungi (AMF) colonize roots, where they provide nutrients in exchange for sugars and lipids. Because AMF lack genes for cytosolic fatty acid de novo synthase (FAS), they depend on host-derived fatty acids. AMF colonization is accompanied by expression of specific lipid genes and synthesis of sn-2 monoacylglycerols (MAGs). It is unknown how host-derived fatty acids are taken up by AMF. We describe the characterization of two AMP-binding domain protein genes from Rhizophagus irregularis, RiFAT1 and RiFAT2, with sequence similarity to Saccharomyces cerevisiae fatty acid transporter 1 (FAT1). Uptake of C-13-myristic acid (14:0) and, to a lesser extent, C-13-palmitic acid (16:0) was enhanced after expression of RiFAT1 or RiFAT2 in S. cerevisiae Afatl cells. The uptake of( 2)H-labeled fatty acids from H-2-myristoylglycerol or 2(H)-pahnitoylglycerol was also increased after RiFAT1 and RiFAT2 expression in Afat, but intact H-2-MAGs were not detected. RiFAT1 and RiFAT2 expression was induced in colonized roots compared with extraradical mycelium. C-13-label in the AMF-specific palmitvaccenic acid (16:1A11) and eicosatrienoic acid (20:3) were detected in colonized roots only when C-13(2)-acetate was supplemented but not C-13-fatty acids, demonstrating that de novo synthesized, host-derived fatty acids are rapidly taken up by R. irregularis from the roots. The results show that RiFAT1 and RiFAT2 are involved in the uptake of myristic acid (14:0) and palmitic acid (16:0), while fatty acids from MAGs are only taken up after hydrolysis. Therefore, the two proteins might be involved in fatty acid import into the fungal arbuscules in colonized roots.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brands, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-665005
DOI:	10.1094/MPMI-01-22-0026-R
Journal or Publication Title:	Mol. Plant-Microbe Interact.
Volume:	35
Number:	6
Page Range:	S. 464 - 477
Date:	2022
Publisher:	AMER PHYTOPATHOLOGICAL SOC
Place of Publication:	ST PAUL
ISSN:	1943-7706
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACYL-COA SYNTHETASE; ARBUSCULAR-MYCORRHIZAL FUNGI; COENZYME-A SYNTHETASE; SACCHAROMYCES-CEREVISIAE; LIPID-METABOLISM; ACETYL-COENZYME; TRANSPORT; GROWTH; PLANT; FAT1P Multiple languages Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66500