Neef, Klaus, Treskes, Philipp, Xu, Guoxing, Drey, Florian, Srinivasan, Sureshkumar Perumal, Saric, Tomo ORCID: 0000-0001-8344-1095, Nembo, Erastus, Semmler, Judith, Nguemo, Filomain, Stamm, Christof, Cowan, Douglas B., Deppe, Antje-Christin, Scherner, Maximilian, Wittwer, Thorsten, Hescheler, Juergen, Wahlers, Thorsten and Choi, Yeong-Hoon (2015). Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro. Stem Cells Int., 2015. LONDON: HINDAWI LTD. ISSN 1687-9678

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Abstract

Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome. Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches. Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells. A subpopulation of nonadherent cells was isolated from skeletal muscle by preplating and applying cell culture conditions differing in support of cluster formation. In contrast to static culture conditions, dynamic culture with or without previous hanging drop preculture led to significantly increased cluster diameters and the expression of cardiac specific markers on the protein and mRNA level. Whole-cell patch-clamp studies revealed similarities to pacemaker action potentials and responsiveness to cardiac specific pharmacological stimuli. This data indicates that skeletal muscle-derived stem cells are capable of adopting enhanced cardiac muscle cell-like properties by applying specific culture conditions. Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Neef, KlausUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Treskes, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xu, GuoxingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drey, FlorianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Srinivasan, Sureshkumar PerumalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saric, TomoUNSPECIFIEDorcid.org/0000-0001-8344-1095UNSPECIFIED
Nembo, ErastusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Semmler, JudithUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nguemo, FilomainUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stamm, ChristofUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cowan, Douglas B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Deppe, Antje-ChristinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scherner, MaximilianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wittwer, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wahlers, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Choi, Yeong-HoonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-415871
DOI: 10.1155/2015/247091
Journal or Publication Title: Stem Cells Int.
Volume: 2015
Date: 2015
Publisher: HINDAWI LTD
Place of Publication: LONDON
ISSN: 1687-9678
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MYOGENIC SATELLITE CELLS; MYOBLAST TRANSPLANTATION; HEART REGENERATION; CARDIOMYOCYTES; DIFFERENTIATION; ENGRAFTMENT; MYOCARDIUM; EXPRESSION; THERAPYMultiple languages
Cell & Tissue EngineeringMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/41587

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