Universität zu Köln

Entila, Frederickson (2024). Immunity-dependency of plant-microbiota dialogues. PhD thesis, Universität zu Köln.

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Abstract

Despite the beneficial effects of plant microbiota, these assemblages also comprise potentially detrimental microbes. How the plant immunity regulates its microbiota to promote plant health under these conditions remains poorly navigated. We found that commensal bacteria isolated from healthy Arabidopsis thaliana plants can induce varying immune responses with which is tailored in a strain-specific manner and does not coincide with their phylogeny. We have revealed that the immune output RBOHD-generated ROS selectively restricted colonization of potentially pathogenic Xanthomonas L148. Through random genome-wide mutagenesis, we found that the Xanthomonas L148 gspE, encoding a type II secretion system (T2SS) structural component, is essential for Xanthomonas L148 to elicit diseases on rbohD mutant plants. In planta bacterial transcriptomics revealed that RBOHD suppresses most carbohydrate-active enzymes (CAZymes) and T2SS gene expression including gspE. Xanthomonas L148 colonization protected plants against a foliar pathogen and its protective function is independent of its pathogenic potential. Thus, plant-derived ROS turns a potentially detrimental leaf commensal into a beneficial bacterium for the host plant by suppressing T2SS.

Item Type:	Thesis (PhD thesis)
Translated title:	Title Language UNSPECIFIED English
Creators:	Creators Email ORCID ORCID Put Code Entila, Frederickson fentila@mpipz.mpg.de UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-735906
Date:	2024
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Außeruniversitäre Forschungseinrichtungen > MPI for Plant Breeding Research
Subjects:	Natural sciences and mathematics Life sciences Agriculture
Uncontrolled Keywords:	Keywords Language pattern triggered immunity English ROS English commensal English
Date of oral exam:	10 August 2023
Referee:	Name Academic Title Schulze-Lefert, Paul Prof. Dr. Kopriva, Stanislav Prof. Dr.
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/73590