Universität zu Köln

Matias, Ana C. ORCID: 0000-0002-6385-9117, Tiago, Margarida N. ORCID: 0000-0001-8992-7141, Zimmermann, Jessica, Dohmen, R. Jürgen ORCID: 0000-0002-5756-6780 and Ramos, Paula C. ORCID: 0009-0005-5007-3894 (2025). The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly. Biomolecules, 15 (5). MDPI. ISSN 2218-273X

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Abstract

[Artikel-Nr.: 683] A complex composed of Pba3-Pba4 and subunits α5, α6, and α7 is identified as an early intermediate in proteasome core particle assembly in wild-type Saccharomyces cerevisiae cells. The same complex can be reconstituted from recombinantly produced components in vitro. Assembly of α6 and α7 with Pba3-Pba4 depends on the presence of the α5 subunit, the binding of which apparently initiates the formation of this intermediate. Our data suggest the following order of events: first, Pba3-Pba4 binds α5, then α6 is incorporated, and at the end α7. In the absence of the chaperones Pba1-Pba2 or Ump1, alternative Pba4-containing complexes are detected, the formation of which depends on the Blm10/PA200 protein. Overexpression of Pba1-Pba2 abolishes the formation of these complexes containing Pba4 and Blm10, suggesting that Blm10 may replace Pba1-Pba2 as an alternative assembly factor.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Matias, Ana C. UNSPECIFIED https://orcid.org/0000-0002-6385-9117 UNSPECIFIED Tiago, Margarida N. UNSPECIFIED https://orcid.org/0000-0001-8992-7141 UNSPECIFIED Zimmermann, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Dohmen, R. Jürgen UNSPECIFIED https://orcid.org/0000-0002-5756-6780 UNSPECIFIED Ramos, Paula C. UNSPECIFIED https://orcid.org/0009-0005-5007-3894 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-799993
Identification Number:	10.3390/biom15050683
Journal or Publication Title:	Biomolecules
Volume:	15
Number:	5
Number of Pages:	19
Date:	8 May 2025
Publisher:	MDPI
ISSN:	2218-273X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	Life sciences
Uncontrolled Keywords:	Keywords Language proteasome core particle ; assembly ; chaperones ; Pba3-Pba4 ; Blm10 English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/79999