Universität zu Köln

Nguemo, Filomain ORCID: 0000-0002-2236-6797, Zhang, Hai ORCID: 0000-0001-6775-1050, Koester, Annette, Rohani, Susan, Perumal Srinivasan, Sureshkumar and Hescheler, Jürgen ORCID: 0000-0001-7686-6562 (2025). A Nanoformulation of Ubiquinol and Selenium Promotes Proliferation of Human Induced Pluripotent Stem Cells. Antioxidants, 14 (9). MDPI. ISSN 2076-3921

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Abstract

[Artikel-Nr. 1100] Human induced pluripotent stem cells (hiPSCs) hold immense promise for regenerative medicine. However, a critical barrier to the clinical application of hiPSCs is the difficulty in promoting robust cell proliferation while preserving their pluripotent state. Efficient hiPSC expansion without loss of pluripotency is crucial for generating high quality cells or therapeutic applications, disease modeling, and drug discovery. In our study, we investigated the effects of QuinoMit Q10® fluid (QMF-Se), a nanoformulated supplement containing Ubiquinol (the active form of Coenzyme Q10) and Selenium, on hiPSC growth and maintenance in vitro. Interesting, QMF-Se supplementation significantly enhances hiPSC proliferation compared to control cultures. This increase in cell number was accompanied by heightened mitochondrial activity, suggesting improved cellular energy metabolism. Importantly, the expression of core pluripotency markers OCT4, NANOG, and SOX2 remained unaltered, confirming that the stem cells retained their undifferentiated status. Moreover, we observed that QMF-Se treatment conferred protective effects during the freeze–thaw process, reducing cell death and supporting post-thaw recovery. These results indicate that QMF-Se may improve both cell culture efficiency and cryopreservation outcomes. Overall, our findings highlight the potential of QMF-Se as a valuable additive for hiPSC culture systems, contributing to more efficient and reliable expansion protocols in regenerative medicine research.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Nguemo, Filomain UNSPECIFIED https://orcid.org/0000-0002-2236-6797 UNSPECIFIED Zhang, Hai UNSPECIFIED https://orcid.org/0000-0001-6775-1050 UNSPECIFIED Koester, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohani, Susan UNSPECIFIED UNSPECIFIED UNSPECIFIED Perumal Srinivasan, Sureshkumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Jürgen UNSPECIFIED https://orcid.org/0000-0001-7686-6562 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-800659
Identification Number:	10.3390/antiox14091100
Journal or Publication Title:	Antioxidants
Volume:	14
Number:	9
Number of Pages:	13
Date:	10 September 2025
Publisher:	MDPI
ISSN:	2076-3921
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Physiologie und Pathophysiologie > Zentrum für Physiologie und Pathophysiologie
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language QuinoMit Q10 fluid ; hiPSCs ; proliferation ; mitochondria ; cryopreservation English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/80065