Universität zu Köln

Tonelotto, Valentina, Trapani, Valeria, Bretaud, Sandrine, Heumueller, Stefanie Elisabeth, Wagener, Raimund, Ruggiero, Florence and Bonaldo, Paolo ORCID: 0000-0002-9571-8140 (2019). Spatio-temporal expression and distribution of collagen VI during zebrafish development. Sci Rep, 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

Full text not available from this repository.

Abstract

Collagen VI (ColVI) is an extracellular matrix (ECM) protein involved in a range of physiological and pathological conditions. Zebrafish (Danio rerio) is a powerful model organism for studying vertebrate development and for in vivo analysis of tissue patterning. Here, we performed a thorough characterization of ColVI gene and protein expression in zebrafish during development and adult life. Bioinformatics analyses confirmed that zebrafish genome contains single genes encoding for alpha 1(VI), alpha 2(VI) and alpha 3(VI) ColVI chains and duplicated genes encoding for alpha 4(VI) chains. At 1 day post-fertilization (dpf) ColVI transcripts are expressed in myotomes, pectoral fin buds and developing epidermis, while from 2 dpf abundant transcript levels are present in myosepta, pectoral fins, axial vasculature, gut and craniofacial cartilage elements. Using newly generated polyclonal antibodies against zebrafish alpha 1(VI) protein, we found that ColVI deposition in adult fish delineates distinct domains in the ECM of several organs, including cartilage, eye, skin, spleen and skeletal muscle. Altogether, these data provide the first detailed characterization of ColVI expression and ECM deposition in zebrafish, thus paving the way for further functional studies in this species.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tonelotto, Valentina UNSPECIFIED UNSPECIFIED UNSPECIFIED Trapani, Valeria UNSPECIFIED UNSPECIFIED UNSPECIFIED Bretaud, Sandrine UNSPECIFIED UNSPECIFIED UNSPECIFIED Heumueller, Stefanie Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruggiero, Florence UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonaldo, Paolo UNSPECIFIED orcid.org/0000-0002-9571-8140 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-123873
DOI:	10.1038/s41598-019-56445-4
Journal or Publication Title:	Sci Rep
Volume:	9
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CONGENITAL MUSCULAR-DYSTROPHY; IN-VIVO; MITOCHONDRIAL DYSFUNCTION; SIGNALING CONTROLS; MUSCLE; CHAINS; AUTOPHAGY; SEQUENCE; GENOME; FISH Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12387