Universität zu Köln

Springer, Jan, Walther, Grit, Rickerts, Volker, Hamprecht, Axel ORCID: 0000-0003-1449-5780, Willinger, Birgit ORCID: 0000-0001-7921-5749, Teschner, Daniel ORCID: 0000-0001-7351-8060, Einsele, Hermann, Kurzai, Oliver and Loeffler, Juergen (2019). Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR. J. Fungi, 5 (4). BASEL: MDPI. ISSN 2309-608X

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Abstract

The mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several different clinical specimens detecting Fusarium spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed (n = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for Fusarium species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of Fusarium species and its application to clinically relevant specimens.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Springer, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Walther, Grit UNSPECIFIED UNSPECIFIED UNSPECIFIED Rickerts, Volker UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED Willinger, Birgit UNSPECIFIED orcid.org/0000-0001-7921-5749 UNSPECIFIED Teschner, Daniel UNSPECIFIED orcid.org/0000-0001-7351-8060 UNSPECIFIED Einsele, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurzai, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeffler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-125397
DOI:	10.3390/jof5040105
Journal or Publication Title:	J. Fungi
Volume:	5
Number:	4
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMMUNOCOMPROMISED PATIENTS; INFECTIONS; EPIDEMIOLOGY; DIAGNOSIS; SPP.; IDENTIFICATION; EMERGENCE; SYSTEM; MOLDS Multiple languages Microbiology; Mycology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12539