Universität zu Köln

Badia, Mariana Beatriz ORCID: 0000-0001-7981-8910, Maurino, Veronica Graciela, Pavlovic, Tatiana, Arias, Cintia Lucia, Pagani, Maria Ayelen ORCID: 0000-0001-9592-3518, Andreo, Carlos Santiago, Saigo, Mariana, Drincovich, Maria Fabiana and Gerrard Wheeler, Mariel Claudia ORCID: 0000-0001-5232-5542 (2020). Loss of function of Arabidopsis NADP-malic enzyme 1 results in enhanced tolerance to aluminum stress. Plant J., 101 (3). S. 653 - 666. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

In acidic soils, aluminum (Al) toxicity is a significant limitation to crop production worldwide. Given its Al-binding capacity, malate allows internal as well as external detoxification strategies to cope with Al stress, but little is known about the metabolic processes involved in this response. Here, we analyzed the relevance of NADP-dependent malic enzyme (NADP-ME), which catalyzes the oxidative decarboxylation of malate, in Al tolerance. Plants lacking NADP-ME1 (nadp-me1) display reduced inhibition of root elongation along Al treatment compared with the wild type (wt). Moreover, wt roots exposed to Al show a drastic decrease in NADP-ME1 transcript levels. Although malate levels in seedlings and root exudates are similar in nadp-me1 and wt, a significant increase in intracellular malate is observed in roots of nadp-me1 after long exposure to Al. The nadp-me1 plants also show a lower H2O2 content in root apices treated with Al and no inhibition of root elongation when exposed to glutamate, an amino acid implicated in Al signaling. Proteomic studies showed several differentially expressed proteins involved in signal transduction, primary metabolism and protection against biotic and other abiotic stimuli and redox processes in nadp-me1, which may participate directly or indirectly in Al tolerance. The results indicate that NADP-ME1 is involved in adjusting the malate levels in the root apex, and its loss results in an increased content of this organic acid. Furthermore, the results suggest that NADP-ME1 affects signaling processes, such as the generation of reactive oxygen species and those that involve glutamate, which could lead to inhibition of root growth.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Badia, Mariana Beatriz UNSPECIFIED orcid.org/0000-0001-7981-8910 UNSPECIFIED Maurino, Veronica Graciela UNSPECIFIED UNSPECIFIED UNSPECIFIED Pavlovic, Tatiana UNSPECIFIED UNSPECIFIED UNSPECIFIED Arias, Cintia Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Pagani, Maria Ayelen UNSPECIFIED orcid.org/0000-0001-9592-3518 UNSPECIFIED Andreo, Carlos Santiago UNSPECIFIED UNSPECIFIED UNSPECIFIED Saigo, Mariana UNSPECIFIED UNSPECIFIED UNSPECIFIED Drincovich, Maria Fabiana UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerrard Wheeler, Mariel Claudia UNSPECIFIED orcid.org/0000-0001-5232-5542 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-127386
DOI:	10.1111/tpj.14571
Journal or Publication Title:	Plant J.
Volume:	101
Number:	3
Page Range:	S. 653 - 666
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENOME-WIDE IDENTIFICATION; OXIDATIVE STRESS; SEED MATURATION; ROOT-GROWTH; GENES; RESPONSES; REVEALS; EXPRESSION; GERMINATION; METABOLISM Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12738