Universität zu Köln

Grevenstein, David ORCID: 0000-0003-4823-7739, Heilig, Juliane, Dargel, Jens, Oppermann, Johannes, Eysel, Peer, Brochhausen, Christoph and Niehoff, Anja ORCID: 0000-0002-4165-0929 (2020). COMP in the Infrapatellar Fat Pad-Results of a Prospective Histological, Immunohistological, and Biochemical Case-Control Study. J. Orthop. Res., 38 (4). S. 747 - 759. HOBOKEN: WILEY. ISSN 1554-527X

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Abstract

Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high-grade OA (mean age 63.8 +/- 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 +/- 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1 beta [IL-1 beta] and IL-6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix-metalloproteinase-3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. (c) 2019 The Authors. Journal of Orthopaedic Research (R) published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Grevenstein, David UNSPECIFIED orcid.org/0000-0003-4823-7739 UNSPECIFIED Heilig, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Dargel, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Oppermann, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Eysel, Peer UNSPECIFIED UNSPECIFIED UNSPECIFIED Brochhausen, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Niehoff, Anja UNSPECIFIED orcid.org/0000-0002-4165-0929 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-127473
DOI:	10.1002/jor.24514
Journal or Publication Title:	J. Orthop. Res.
Volume:	38
Number:	4
Page Range:	S. 747 - 759
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1554-527X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OLIGOMERIC MATRIX PROTEIN; MESENCHYMAL STEM-CELLS; KNEE OSTEOARTHRITIS; ADIPOSE-TISSUE; SERUM MARKERS; COLLAGEN-II; CARTILAGE; LEPTIN; BONE; INFLAMMATION Multiple languages Orthopedics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12747