Universität zu Köln

Maleckova, Eva ORCID: 0000-0001-9062-3516, Brilhaus, Dominik ORCID: 0000-0001-9021-3197, Wrobel, Thomas J. and Weber, Andreas P. M. (2019). Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare. J. Exp. Bot., 70 (22). S. 6581 - 6597. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

Crassulacean acid metabolism (CAM) has evolved as a water-saving strategy, and its engineering into crops offers an opportunity to improve their water use efficiency. This requires a comprehensive understanding of the regulation of the CAM pathway. Here, we use the facultative CAM species Talinum triangulare as a model in which CAM can be induced rapidly by exogenous abscisic acid. RNA sequencing and metabolite measurements were employed to analyse the changes underlying CAM induction and identify potential CAM regulators. Non-negative matrix factorization followed by k-means clustering identified an early CAM-specific cluster and a late one, which was specific for the early light phase. Enrichment analysis revealed abscisic acid metabolism, WRKY-regulated transcription, sugar and nutrient transport, and protein degradation in these clusters. Activation of the CAM pathway was supported by up-regulation of phosphoenolpyruvate carboxylase, cytosolic and chloroplastic malic enzymes, and several transport proteins, as well as by increased end-of-night titratable acidity and malate accumulation. The transcription factors HSFA2, NF-YA9, and JMJ27 were identified as candidate regulators of CAM induction. With this study we promote the model species T. triangulare, in which CAM can be induced in a controlled way, enabling further deciphering of CAM regulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Maleckova, Eva UNSPECIFIED orcid.org/0000-0001-9062-3516 UNSPECIFIED Brilhaus, Dominik UNSPECIFIED orcid.org/0000-0001-9021-3197 UNSPECIFIED Wrobel, Thomas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-127499
DOI:	10.1093/jxb/erz189
Journal or Publication Title:	J. Exp. Bot.
Volume:	70
Number:	22
Page Range:	S. 6581 - 6597
Date:	2019
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HALOPHYTE MESEMBRYANTHEMUM-CRYSTALLINUM; METHIONINE GAMMA-LYASE; DAY-NIGHT CHANGES; FREE AMINO-ACIDS; PHOSPHOENOLPYRUVATE CARBOXYLASE; STRESS TOLERANCE; CAM PLANT; STARCH DEGRADATION; GLYCINE BETAINE; ANANAS-COMOSUS Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12749