Liu, Pei Y., Tee, Andrew E., Milazzo, Giorgio, Hannan, Katherine M., Maag, Jesper ORCID: 0000-0002-2578-8872, Mondal, Sujanna, Atmadibrata, Bernard, Bartonicek, Nenad, Peng, Hui, Ho, Nicholas, Mayoh, Chelsea, Ciaccio, Roberto, Sun, Yuting, Henderson, Michelle J., Gao, Jixuan, Everaert, Celine ORCID: 0000-0001-7772-4259, Hulme, Amy J., Wong, Matthew ORCID: 0000-0003-2680-961X, Lan, Qing, Cheung, Belamy B., Shi, Leming, Wang, Jenny Y., Simon, Thorsten, Fischer, Matthias, Zhang, Xu D., Marshall, Glenn M., Norris, Murray D., Haber, Michelle, Vandesompele, Jo, Li, Jinyan, Mestdagh, Pieter, Hannan, Ross D., Dinger, Marcel E., Perini, Giovanni and Liu, Tao ORCID: 0000-0001-6244-7316 (2019). The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35. Nat. Commun., 10. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

Full text not available from this repository.

Abstract

The majority of patients with neuroblastoma due to MYCN oncogene amplification and consequent N-Myc oncoprotein over-expression die of the disease. Here our analyses of RNA sequencing data identify the long noncoding RNA lncNB1 as one of the transcripts most overexpressed in MYCN-amplified, compared with MYCN-non-amplified, human neuroblastoma cells and also the most over-expressed in neuroblastoma compared with all other cancers. lncNB1 binds to the ribosomal protein RPL35 to enhance E2F1 protein synthesis, leading to DEPDC1B gene transcription. The GTPase-activating protein DEPDC1B induces ERK protein phosphorylation and N-Myc protein stabilization. Importantly, lncNB1 knockdown abolishes neuroblastoma cell clonogenic capacity in vitro and leads to neuroblastoma tumor regression in mice, while high levels of lncNB1 and RPL35 in human neuroblastoma tissues predict poor patient prognosis. This study therefore identifies lncNB1 and its binding protein RPL35 as key factors for promoting E2F1 protein synthesis, N-Myc protein stability and N-Myc-driven oncogenesis, and as therapeutic targets.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Liu, Pei Y.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tee, Andrew E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Milazzo, GiorgioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hannan, Katherine M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maag, JesperUNSPECIFIEDorcid.org/0000-0002-2578-8872UNSPECIFIED
Mondal, SujannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Atmadibrata, BernardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bartonicek, NenadUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peng, HuiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ho, NicholasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mayoh, ChelseaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ciaccio, RobertoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sun, YutingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Henderson, Michelle J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gao, JixuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Everaert, CelineUNSPECIFIEDorcid.org/0000-0001-7772-4259UNSPECIFIED
Hulme, Amy J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wong, MatthewUNSPECIFIEDorcid.org/0000-0003-2680-961XUNSPECIFIED
Lan, QingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cheung, Belamy B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shi, LemingUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, Jenny Y.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Simon, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fischer, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, Xu D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marshall, Glenn M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Norris, Murray D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haber, MichelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vandesompele, JoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, JinyanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mestdagh, PieterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hannan, Ross D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dinger, Marcel E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Perini, GiovanniUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liu, TaoUNSPECIFIEDorcid.org/0000-0001-6244-7316UNSPECIFIED
URN: urn:nbn:de:hbz:38-128220
DOI: 10.1038/s41467-019-12971-3
Journal or Publication Title: Nat. Commun.
Volume: 10
Date: 2019
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
GENE-EXPRESSION; BIOCONDUCTOR PACKAGE; MYC; NEUROBLASTOMA; CHROMATIN; TARGETMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/12822

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item