Universität zu Köln

Seidl, Elias, Kiermeier, Hannah, Liebisch, Gerhard ORCID: 0000-0003-4886-0811, Ballmann, Manfred, Hesse, Sebastian, Paul-Buck, Karl, Ratjen, Felix, Rietscherl, Ernst and Griese, Matthias (2019). Lavage lipidomics signatures in children with cystic fibrosis and protracted bacterial bronchitis. J. Cyst. Fibros, 18 (6). S. 790 - 796. AMSTERDAM: ELSEVIER. ISSN 1873-5010

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Abstract

Background: Balanced composition of a well-functioning pulmonary surfactant is crucial and essential for normal breathing. Here, we explored whether the composition of lipids recovered by broncho-alveolar lavage (BAL) in children with cystic fibrosis (CF) differ from children with protracted bacterial bronchitis (PBB) and controls. We wanted to differentiate, if alterations are primarily caused by the disease process or secondary due to an increased amount of cell-membrane lipids derived from inflammatory cells. Methods: Comprehensive lipidomics profiles of BAL fluid from children diagnosed with CF, PBB and controls were generated by electrospray ionization tandem mass spectrometry analysis. BAL cell differential and numbers were examined. Results: 55 children (37 patients with CF, 8 children with PBB and 10 controls) were included in this study. Results showed comparable total quantities of lipids in all groups. Phospholipids were the major lipid fraction and similar in all groups, whereas the fractions of cholesteryl esters were less and of free cholesterol were increased in children with CF. Among the phospholipids, patients with CF had higher proportion of the non-surfactant membrane-lipids in the classes phosphatidylethanolamine based plasmalogens (PE P), phosphatidylethanolmine (PE) and phosphatidylserine (PS), but a lower proportion of phosphatidylcholine (PC) compared to healthy controls. No such changes were identified in the BAL fluid of children diagnosed with PBB. No differences were observed for the surfactant lipids dipalmitoyl-phosphatidylcholin (PC 32:0) and phosphatidylglycerol (PG). Conclusions: In CF patients with neutrophilic airway inflammation the lipid composition for surfactant phospholipid components were unchanged, whereas alteration in lipid profile were characteristic for those found in membranes of inflammatory cells. We suspect that the changes in CF were caused by the prolonged inflammation in contrast to a relatively short standing process in PBB. (C) 2019 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Seidl, Elias UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiermeier, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Liebisch, Gerhard UNSPECIFIED orcid.org/0000-0003-4886-0811 UNSPECIFIED Ballmann, Manfred UNSPECIFIED UNSPECIFIED UNSPECIFIED Hesse, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Paul-Buck, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Ratjen, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Rietscherl, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED Griese, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-128553
DOI:	10.1016/j.jcf.2019.04.012
Journal or Publication Title:	J. Cyst. Fibros
Volume:	18
Number:	6
Page Range:	S. 790 - 796
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-5010
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; CHRONIC WET COUGH; PULMONARY SURFACTANT; LUNG-FUNCTION; PHOSPHOLIPID-COMPOSITION; ARACHIDONIC-ACID; YOUNG-CHILDREN; INFLAMMATION; DISEASE Multiple languages Respiratory System Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12855