Universität zu Köln

Notara, Maria, Lentzsch, Anna, Clahsen, Thomas, Behboudifard, Sara, Braun, Gabriele and Cursiefen, Claus (2019). Bevacizumab Induces Upregulation of Keratin 3 and VEGFA in Human Limbal Epithelial Cells in Vitro. J. Clin. Med., 8 (11). BASEL: MDPI. ISSN 2077-0383

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Abstract

Topical application of vascular endothelial growth factor A (VEGFA) inhibitors including Bevacizumab is used for antiangiogenic therapy at the ocular surface. While clinical studies have suggested that this approach is well-tolerated, the effect of the drug on limbal epithelial stem cells has not been studied. In this study, the effect of Bevacizumab on phenotype and functionality of putative limbal epithelial stem cells (SC) was investigated. The effect of Bevacizumab on human limbal epithelial cells was assessed in terms of metabolic activity and scratch wound closure. The different treatment groups featured no difference in proliferation and colony forming efficiency (CFE) of limbal epithelial cells or their putative SC marker expression. A significant delay in scratch closure of all the Bevacizumab-treated groups was detected at 4 h. RNA and protein quantification indicated a dose-responsive increase of keratin 3. VEGFA RNA expression also increased while VEGFC and D as well as VEGFR1, 2 and 3 were unchanged. This study highlights previously unknown effects of Bevacizumab on cultured putative limbal epithelial SC: a dose-related increase of keratin 3, an increase in VEGFA as well as a delay in scratch wound closure. These in vitro data should be considered when using Bevacizumab in the context of limbal epithelial SC transplantation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Notara, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Lentzsch, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Clahsen, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Behboudifard, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Braun, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-128769
DOI:	10.3390/jcm8111925
Journal or Publication Title:	J. Clin. Med.
Volume:	8
Number:	11
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2077-0383
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CORNEAL NEOVASCULARIZATION; SAFETY PROFILE; EYE DROPS; AVASTIN; EXPRESSION; THERAPY; DIFFERENTIATION; AVASCULARITY Multiple languages Medicine, General & Internal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12876