Universität zu Köln

Tiwari, Manu D., Zeitler, Daniela M., Meister, Gunter and Wodarz, Andreas ORCID: 0000-0002-0007-6289 (2019). Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation. Biol. Open, 8 (11). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 2046-6390

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Abstract

Stem cells can self-renew and produce daughter cells destined for differentiation. The precise control of the balance between these two outcomes is essential to ensure tissue homeostasis and to prevent uncontrolled proliferation resulting in tumor formation. As self-renewal and differentiation are likely to be controlled by different gene expression programs, unraveling the underlying gene regulatory networks is crucial for understanding the molecular logic of this system. In this study, we have characterized by next generation RNA sequencing (RNA-seq) the transcriptome of germline stem cell (GSC)-like cells isolated from bag of marbles (bam) mutant Drosophila ovaries and compared it to the transcriptome of germ line cells isolated from wild-type ovaries. We have complemented this dataset by utilizing an RNA-immunoprecipitation strategy to identify transcripts bound to the master differentiation factor Bam. Protein complex enrichment analysis on these combined datasets allows us to delineate known and novel networks essential for GSC maintenance and differentiation. Further comparative transcriptomics illustrates similarities between GSCs and primordial germ cells and provides a molecular footprint of the stem cell state. Our study represents a useful resource for functional studies on stem cell maintenance and differentiation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tiwari, Manu D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeitler, Daniela M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Meister, Gunter UNSPECIFIED UNSPECIFIED UNSPECIFIED Wodarz, Andreas UNSPECIFIED orcid.org/0000-0002-0007-6289 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-128852
DOI:	10.1242/bio.046789
Journal or Publication Title:	Biol. Open
Volume:	8
Number:	11
Date:	2019
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2046-6390
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BAG-OF-MARBLES; SELF-RENEWAL; TRANSLATIONAL CONTROL; ASYMMETRIC DIVISION; TRANSGENIC RNAI; CYST FORMATION; SEX-LETHAL; NIPPED-A; GENE; MAINTENANCE Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12885