Universität zu Köln

Ryll, Martin, Bucher, Julian, Drefs, Moritz, Boesch, Florian, Kumaraswami, K., Schiergens, Tobias, Niess, Hanno, Schoenberg, Markus, Jacob, Sven, Rentsch, Markus, Guba, Markus, Werner, Jens, Andrassy, Joachim and Thomas, Michael N. (2019). Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique. J. Vis. Exp. (153). CAMBRIDGE: JOURNAL OF VISUALIZED EXPERIMENTS. ISSN 1940-087X

Full text not available from this repository.

Abstract

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. However, only minor improvement in the long-term results of transplanted solid organs could be observed over the past decades. In this context, chronic allograft vasculopathy (CAV) still represents the leading cause of late organ failure in cardiac, renal and pulmonary transplantation. Thus far, the underlying pathogenesis of CAV development remains unclear, explaining why effective treatment strategies are presently missing and emphasizing a need for relevant experimental models in order to study the underlying pathophysiology leading to CAV formation. The following protocol describes a murine heterotopic cervical aortic transplantation model using a modified non-suture cuff technique. In this technique, a segment of the thoracic aorta is interpositioned in the right common carotid artery. With the use of the non-suture cuff technique, an easy to learn and reproducible model can be established, minimizing the possible heterogeneity of sutured vascular micro anastomoses.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ryll, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucher, Julian UNSPECIFIED UNSPECIFIED UNSPECIFIED Drefs, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Boesch, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumaraswami, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiergens, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Niess, Hanno UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoenberg, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Jacob, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Rentsch, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Guba, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Werner, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Andrassy, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Michael N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-128942
DOI:	10.3791/59983
Journal or Publication Title:	J. Vis. Exp.
Number:	153
Date:	2019
Publisher:	JOURNAL OF VISUALIZED EXPERIMENTS
Place of Publication:	CAMBRIDGE
ISSN:	1940-087X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CARDIAC ALLOGRAFT VASCULOPATHY; MOUSE; SURVIVAL Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12894