Universität zu Köln

Eom, Joon-Seob, Luo, Dangping, Atienza-Grande, Genelou, Yang, Jungil, Ji, Chonghui, Huguet-Tapia, Jose C., Char, Si Nian, Liu, Bo, Nguyen, Hanna, Schmidt, Sarah Maria, Szurek, Boris, Cruz, Casiana Vera, White, Frank F., Oliva, Ricardo, Yang, Bing ORCID: 0000-0002-2293-3384 and Frommer, Wolf B. (2019). Diagnostic kit for rice blight resistance. Nat. Biotechnol., 37 (11). S. 1372 - 1381. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1546-1696

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Abstract

Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT-PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR-Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant 'mega' rice lines that will empower farmers to plant lines that are most likely to resist rice blight.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eom, Joon-Seob UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Dangping UNSPECIFIED UNSPECIFIED UNSPECIFIED Atienza-Grande, Genelou UNSPECIFIED UNSPECIFIED UNSPECIFIED Yang, Jungil UNSPECIFIED UNSPECIFIED UNSPECIFIED Ji, Chonghui UNSPECIFIED UNSPECIFIED UNSPECIFIED Huguet-Tapia, Jose C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Char, Si Nian UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Bo UNSPECIFIED UNSPECIFIED UNSPECIFIED Nguyen, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Sarah Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Szurek, Boris UNSPECIFIED UNSPECIFIED UNSPECIFIED Cruz, Casiana Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED White, Frank F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oliva, Ricardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Yang, Bing UNSPECIFIED orcid.org/0000-0002-2293-3384 UNSPECIFIED Frommer, Wolf B. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-129464
DOI:	10.1038/s41587-019-0268-y
Journal or Publication Title:	Nat. Biotechnol.
Volume:	37
Number:	11
Page Range:	S. 1372 - 1381
Date:	2019
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1546-1696
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DISEASE-SUSCEPTIBILITY GENE; BACTERIAL-BLIGHT; DNA; EXPRESSION; INDUCTION; VARIETIES; VIRULENCE; TALEN Multiple languages Biotechnology & Applied Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/12946