Universität zu Köln

Heger, Lukas Andreas, Hortmann, Marcus, Albrecht, Madlin, Colberg, Christian, Peter, Karlheinz, Witsch, Thilo, Stallmann, Daniela, Zirlik, Andreas, Bode, Christoph, Duerschmied, Daniel and Ahrens, Ingo (2019). Inflammation in acute coronary syndrome: Expression of TLR2 mRNA is increased in platelets of patients with ACS. PLoS One, 14 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Background Platelets are key components in atherogenesis and determine the course of its clinical sequelae acute coronary syndrome (ACS). Components of the innate immune system-the superfamily of TLR receptors-are present in platelets and represent a link between atherothrombosis and inflammation. We hypothesize that alteration in platelet TLR mRNA expression is a result of inflammation driving coronary atherosclerosis and may represent an alternative platelet activation pathway in ACS. TLR2-, TLR4- and TLR9-mRNA-expression was determined in ACS patients and compared to patients with invasive exclusion of atherosclerotic lesions of coronary arteries. Methods A total of fifty-four patients were enrolled in this clinical retrospective cohort single centre study. Total RNA from sepharose-filtered highly purified platelets was isolated using acid guanidinium thiocyanate-phenol-chloroform extraction and transcribed to cDNA using a first strand cDNA synthesis kit. To determine absolute copy numbers of TLR2, TLR4 and TLR9 we used plasmid based quantitative PCR with normalisation to an internal control. Results We found that mRNA expression levels of TLR2 but not TLR 4 and 9 are up-regulated in platelets of patients with ACS when compared to patients without coronary atherosclerosis. Conclusion Our results suggest elevated TLR2 mRNA expression in platelets as a biomarker reflecting the underlying inflammation in ACS and possibly severity of coronary atherosclerosis. Platelet TLR2 may represent a link between inflammation and atherothrombosis in ACS.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Heger, Lukas Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hortmann, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED Albrecht, Madlin UNSPECIFIED UNSPECIFIED UNSPECIFIED Colberg, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Peter, Karlheinz UNSPECIFIED UNSPECIFIED UNSPECIFIED Witsch, Thilo UNSPECIFIED UNSPECIFIED UNSPECIFIED Stallmann, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Zirlik, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Bode, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Duerschmied, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Ahrens, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-130466
DOI:	10.1371/journal.pone.0224181
Journal or Publication Title:	PLoS One
Volume:	14
Number:	10
Date:	2019
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TOLL-LIKE RECEPTORS; MYOCARDIAL-INFARCTION; ACTIVATION; LIGANDS; MICE; TOLL-LIKE-RECEPTOR-4; LIPOPOLYSACCHARIDE; ATHEROSCLEROSIS; RECOGNITION; REPERFUSION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13046