Universität zu Köln

Laffer, Bjoern, Bauer, Dirk, Wasmuth, Susanne, Busch, Martin, Jalilvand, Tida Viola, Thanos, Solon, Zu Hoerste, Gerd Meyer, Loser, Karin, Langmann, Thomas, Heiligenhaus, Arnd and Kasper, Maren (2019). Loss of IL-10 Promotes Differentiation of Microglia to a M1 Phenotype. Front. Cell. Neurosci., 13. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1662-5102

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Abstract

Microglia represent the primary resident immune cells of the central nervous system (CNS) and modulate local immune responses. Depending on their physiological functions, microglia can be classified into pro- (M1) and anti-inflammatory (M2) phenotype. Interleukin (IL)-10 is an important modulator of neuronal homeostasis, with anti-inflammatory and neuroprotective functions, and can be released by microglia. Here, we investigated how IL-10 deficiency affected the M1/2 polarization of primary microglia upon lipopolysaccharide (LPS) stimulation in vitro. Microglia phenotypes were analyzed via flow cytometry. Cytokine and chemokine secretion were examined by ELISA and bead-based multiplex LEGENDplex (TM). Our results showed that genetic depletion of IL-10 led to elevated M1 like phenotype (CD86+ CD206-) under pro-inflammatory conditions associated with increased frequency of IL-6+, TNF-alpha+ cells and enhanced release of several pro-inflammatory chemokines. Absence of IL-10 led to an attenuated M2 like phenotype (CD86- CD206+) and a reduced secretion of TGF-beta 1 upon LPS stimulation. In conclusion, IL-10 deficiency may promote the polarization of microglia into M1-prone phenotype under pro-inflammatory conditions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Laffer, Bjoern UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauer, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED Wasmuth, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Busch, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Jalilvand, Tida Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Thanos, Solon UNSPECIFIED UNSPECIFIED UNSPECIFIED Zu Hoerste, Gerd Meyer UNSPECIFIED UNSPECIFIED UNSPECIFIED Loser, Karin UNSPECIFIED UNSPECIFIED UNSPECIFIED Langmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Heiligenhaus, Arnd UNSPECIFIED UNSPECIFIED UNSPECIFIED Kasper, Maren UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-131175
DOI:	10.3389/fncel.2019.00430
Journal or Publication Title:	Front. Cell. Neurosci.
Volume:	13
Date:	2019
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1662-5102
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; MONOCYTE CHEMOATTRACTANT PROTEIN-1; CENTRAL-NERVOUS-SYSTEM; TUMOR-NECROSIS-FACTOR; ACTIVATED MICROGLIA; INTERLEUKIN-10-DEFICIENT MICE; ALTERNATIVE ACTIVATION; ANTIGEN PRESENTATION; P2Y(12) RECEPTOR; GLIAL-CELLS Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13117