Universität zu Köln

Etich, Julia ORCID: 0000-0003-3238-6692, Koch, Manuel, Wagener, Raimund, Zaucke, Frank ORCID: 0000-0002-7680-9354, Fabri, Mario and Brachvogel, Bent (2019). Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing. Int. J. Mol. Sci., 20 (20). BASEL: MDPI. ISSN 1422-0067

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Abstract

The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components. In this study, we used transcriptome profiling and bioinformatic analysis to define the unique expression of ECM-associated genes in cultured macrophages. Characterization of the matrisome revealed that most genes were constitutively expressed and that several genes were uniquely regulated upon interferon gamma (IFN gamma) and dexamethasone stimulation. Among those core matrisome and matrisome-associated components transforming growth factor beta (TGF beta)-induced, matrix metalloproteinase 9 (MMP9), elastin microfibril interfacer (EMILIN)-1, netrin-1 and gliomedin were also present within the wound bed at time points that are characterized by profound macrophage infiltration. Hence, macrophages are a source of ECM components in vitro as well as during skin wound healing, and identification of these matrisome components is a first step to understand the role and therapeutic value of ECM components in macrophages and during wound healing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Etich, Julia UNSPECIFIED orcid.org/0000-0003-3238-6692 UNSPECIFIED Koch, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED orcid.org/0000-0002-7680-9354 UNSPECIFIED Fabri, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-131479
DOI:	10.3390/ijms20205086
Journal or Publication Title:	Int. J. Mol. Sci.
Volume:	20
Number:	20
Date:	2019
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1422-0067
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IV COLLAGEN; STRUCTURAL BASIS; CELL-ADHESION; VI COLLAGEN; IN-VITRO; PERLECAN; IDENTIFICATION; INTEGRINS; ENDOSTATIN; FIBRILLOGENESIS Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13147