Universität zu Köln

Javanmard, Ehsan, Rahimi, Hanieh Mohammad, Niyyati, Maryam, Aghdaei, Hamid Asadzadeh, Sharifdini, Meysam, Mirjalali, Hamed, Zali, Mohammad Reza and Karanis, Panagiotis (2019). Molecular analysis of Blastocystis sp. and its subtypes from treated wastewater routinely used for irrigation of vegetable farmlands in Iran. J. Water Health, 17 (5). S. 837 - 845. LONDON: IWA PUBLISHING. ISSN 1477-8920

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Abstract

Treated wastewater samples were collected, filtered using sterile 47-mm cellulose nitrate membrane and DNA extracted from the filtered materials. The presence of Blastocystis sp. was confirmed via polymerase chain reaction (PCR) targeting the SSU rRNA gene of Blastocystis sp. in 5/12 of samples. Based on the subtype analysis after sequencing, 2, 2 and 1 of ST2, ST6 and ST8 were detected among the isolates, respectively. Furthermore, both ST6s were allele 139, alleles 11 and 138 were identified in ST2 and the only ST8 was allele 95. The phylogenetic tree showed that one of ST2 was clustered together with those ST2 that were already reported from humans and animals. The presence of Blastocystis sp. in treated wastewater can indicate the potential role of this type of water for irrigation in the transmission of pathogenic microorganisms to downstream farmlands.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Javanmard, Ehsan UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Hanieh Mohammad UNSPECIFIED UNSPECIFIED UNSPECIFIED Niyyati, Maryam UNSPECIFIED UNSPECIFIED UNSPECIFIED Aghdaei, Hamid Asadzadeh UNSPECIFIED UNSPECIFIED UNSPECIFIED Sharifdini, Meysam UNSPECIFIED UNSPECIFIED UNSPECIFIED Mirjalali, Hamed UNSPECIFIED UNSPECIFIED UNSPECIFIED Zali, Mohammad Reza UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-132285
DOI:	10.2166/wh.2019.045
Journal or Publication Title:	J. Water Health
Volume:	17
Number:	5
Page Range:	S. 837 - 845
Date:	2019
Publisher:	IWA PUBLISHING
Place of Publication:	LONDON
ISSN:	1477-8920
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GEOGRAPHIC-DISTRIBUTION; PROTOZOAN PARASITES; DRINKING-WATER; TRANSMISSION; INFECTION; SAMPLES; IDENTIFICATION; INDIVIDUALS; COMMUNITY; OUTBREAKS Multiple languages Environmental Sciences; Public, Environmental & Occupational Health; Microbiology; Water Resources Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13228