Universität zu Köln

Huber, Catharina, V, Jakobs, Barbara D., Mishra, Laxmi S., Niedermaier, Stefan, Stift, Marc, Winter, Gudrun, Adamska, Iwona, Funk, Christiane, Huesgen, Pitter F. and Funck, Dietmar (2019). DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis. J. Exp. Bot., 70 (19). S. 5423 - 5437. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

Maintaining mitochondrial proteome integrity is especially important under stress conditions to ensure a continued ATP supply for protection and adaptation responses in plants. Deg/HtrA proteases are important factors in the cellular protein quality control system, but little is known about their function in mitochondria. Here we analyzed the expression pattern and physiological function of Arabidopsis thaliana DEG10, which has homologs in all photosynthetic eukaryotes. Both expression of DEG10:GFP fusion proteins and immunoblotting after cell fractionation showed an unambiguous subcellular localization exclusively in mitochondria. DEG10 promoter:GUS fusion constructs showed that DEG10 is expressed in trichomes but also in the vascular tissue of roots and aboveground organs. DEG10 loss-of-function mutants were impaired in root elongation, especially at elevated temperature. Quantitative proteome analysis revealed concomitant changes in the abundance of mitochondrial respiratory chain components and assembly factors, which partially appeared to depend on altered mitochondrial retrograde signaling. Under field conditions, lack of DEG10 caused a decrease in seed production. Taken together, our findings demonstrate that DEG10 affects mitochondrial proteostasis, is required for optimal root development and seed set under challenging environmental conditions, and thus contributes to stress tolerance of plants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Huber, Catharina, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakobs, Barbara D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mishra, Laxmi S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Niedermaier, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Stift, Marc UNSPECIFIED UNSPECIFIED UNSPECIFIED Winter, Gudrun UNSPECIFIED UNSPECIFIED UNSPECIFIED Adamska, Iwona UNSPECIFIED UNSPECIFIED UNSPECIFIED Funk, Christiane UNSPECIFIED UNSPECIFIED UNSPECIFIED Huesgen, Pitter F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Funck, Dietmar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-132294
DOI:	10.1093/jxb/erz294
Journal or Publication Title:	J. Exp. Bot.
Volume:	70
Number:	19
Page Range:	S. 5423 - 5437
Date:	2019
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COMPUTATIONAL PLATFORM; PROTEIN; CHLOROPLAST; EXPRESSION; PROTEASES; RESPONSES; MEMBRANE; DATABASE; STRESS; AUXIN Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13229