Universität zu Köln

Abraham, Jella-Andrea, Linnartz, Christina, Dreissen, Georg, Springer, Ronald, Blaschke, Stefan, Rueger, Maria A., Fink, Gereon R. ORCID: 0000-0002-8230-1856, Hoffmann, Bernd ORCID: 0000-0002-3803-8835 and Merkel, Rudolf ORCID: 0000-0003-3178-3282 (2019). Directing Neuronal Outgrowth and Network Formation of Rat Cortical Neurons by Cyclic Substrate Stretch. Langmuir, 35 (23). S. 7423 - 7432. WASHINGTON: AMER CHEMICAL SOC. ISSN 0743-7463

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Abstract

Neuronal mechanobiology plays a vital function in brain development and homeostasis with an essential role in neuronal maturation, pathfinding, and differentiation but is also crucial for understanding brain pathology. In this study, we constructed an in vitro system to assess neuronal responses to cyclic strain as a mechanical signal. The selected strain amplitudes mimicked physiological as well as pathological conditions. By subjecting embryonic neuronal cells to cyclic uniaxial strain we could steer the direction of neuronal outgrowth perpendicular to strain direction for all applied amplitudes. A long-term analysis proved maintained growth direction. Moreover, stretched neurons showed an enhanced length, growth, and formation of nascent side branches with most elevated growth rates subsequent to physiological straining. Application of cyclic strain to already formed neurites identified retraction bulbs with destabilized microtubule structures as spontaneous responses. Importantly, neurons were able to adapt to the mechanical signals without induction of cell death and showed a triggered growth behavior when compared to unstretched neurons. The data suggest that cyclic strain plays a critical role in neuronal development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Abraham, Jella-Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Linnartz, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Dreissen, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Springer, Ronald UNSPECIFIED UNSPECIFIED UNSPECIFIED Blaschke, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Rueger, Maria A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Gereon R. UNSPECIFIED orcid.org/0000-0002-8230-1856 UNSPECIFIED Hoffmann, Bernd UNSPECIFIED orcid.org/0000-0002-3803-8835 UNSPECIFIED Merkel, Rudolf UNSPECIFIED orcid.org/0000-0003-3178-3282 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-137881
DOI:	10.1021/acs.langmuir.8b02003
Journal or Publication Title:	Langmuir
Volume:	35
Number:	23
Page Range:	S. 7423 - 7432
Date:	2019
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	0743-7463
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SRC FAMILY KINASE; VISCOELASTIC PROPERTIES; DYNAMIC REORIENTATION; CELLS; GROWTH; MICROTUBULES; STRESS; MECHANICS; PROTEIN; AXONS Multiple languages Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13788