Universität zu Köln

Mahajan, Aparna, Grueneboom, Anika, Petru, Lenka, Podolska, Malgorzata J., Kling, Lasse ORCID: 0000-0003-1246-8228, Maueroeder, Christian, Dahms, Florian, Christiansen, Silke, Guenter, Lochnit, Krenn, Veit, Juenemann, Anselm, Bock, Felix, Schauer, Christine, Schett, Georg, Hohberger, Bettina, Herrmann, Martin ORCID: 0000-0002-0258-2484 and Munoz, Luis E. (2019). Frontline Science: Aggregated neutrophil extracellular traps prevent inflammation on the neutrophil-rich ocular surface. J. Leukoc. Biol., 105 (6). S. 1087 - 1099. HOBOKEN: WILEY. ISSN 1938-3673

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Abstract

Eye rheum is a physiological discharge, which accumulates at the medial angle of the healthy eye soon after opening in the morning. Microscopic evaluation of eye rheum revealed the presence of viable neutrophils, bacteria, epithelial cells, and particles, aggregated by neutrophil extracellular traps. We observed that in the evening, during eye closure, high C5a recruited neutrophils to the tear film and activated them. In this hypoxic area rich in CO2, neutrophils fight microbial aggressors by degranulation. Immediately after eye opening, the microenvironment of the ocular surface changes, the milieu gets normoxic, and loss of CO2 induces subtle alkalinization of tear film. These conditions favored the formation of neutrophil extracellular traps (NETs) that initially covers the ocular surface and tend to aggregate by eyelid blinking. These aggregated neutrophil extracellular traps (aggNETs) are known as eye rheum and contain several viable neutrophils, epithelial cells, dust particles, and crystals packed together by NETs. Similar to aggNETs induced by monosodium urate crystals, the eye rheum shows a robust proteolytic activity that degraded inflammatory mediators before clinically overt inflammation occur. Finally, the eye rheum passively floats with the tear flow to the medial angle of the eye for disposal. We conclude that the aggNETs-based eye rheum promotes cleaning of the ocular surface and ameliorates the inflammation on the neutrophil-rich ocular surfaces.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mahajan, Aparna UNSPECIFIED UNSPECIFIED UNSPECIFIED Grueneboom, Anika UNSPECIFIED UNSPECIFIED UNSPECIFIED Petru, Lenka UNSPECIFIED UNSPECIFIED UNSPECIFIED Podolska, Malgorzata J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kling, Lasse UNSPECIFIED orcid.org/0000-0003-1246-8228 UNSPECIFIED Maueroeder, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Dahms, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Christiansen, Silke UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenter, Lochnit UNSPECIFIED UNSPECIFIED UNSPECIFIED Krenn, Veit UNSPECIFIED UNSPECIFIED UNSPECIFIED Juenemann, Anselm UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Schauer, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schett, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hohberger, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Herrmann, Martin UNSPECIFIED orcid.org/0000-0002-0258-2484 UNSPECIFIED Munoz, Luis E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-138671
DOI:	10.1002/JLB.HI0718-249RR
Journal or Publication Title:	J. Leukoc. Biol.
Volume:	105
Number:	6
Page Range:	S. 1087 - 1099
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1938-3673
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CORNEAL EPITHELIAL-CELLS; IMMUNE-RESPONSE; INTERLEUKIN-8; PH; ELASTASE; RELEASE; DEATH; TEARS; CD43; DEGRANULATION Multiple languages Cell Biology; Hematology; Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13867