Universität zu Köln

Farowski, Fedja, Els, Gregor, Tsakmaklis, Anastasia, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Kahlert, Christian R. ORCID: 0000-0002-0784-3276, Stein-Thoeringer, Christoph K., Bobardt, Johanna S., Dettmer-Wilde, Katja, Oefner, Peter J., Vehreschild, Jorg Janne and Vehreschild, Maria J. G. T. (2019). Assessment of urinary 3-indoxyl sulfate as a marker for gut microbiota diversity and abundance of Clostridiales. Gut Microbes, 10 (2). S. 133 - 142. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1949-0984

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Abstract

Objectives: After allogeneic hematopoietic stem cell transplantation (allo-HCT), urinary levels of 3-indoxyl sulfate (3-IS) correlate with the relative abundance of bacteria from the class Clostridia (RAC), and antibiotic treatment is considered the major determinant of this outcome. A high RAC has been associated with favorable outcome after allo-HCT and protection from Clostridium difficile infection (CDI). We assessed correlations between alpha diversity, RAC and urinary 3-IS levels in a non-allo-HCT clinical cohort of antibiotic treated patients to further explore 3-IS as a biomarker of reduced diversity and predisposition to CDI. Methods: Fecal and urinary specimens were analyzed from 40 non-alto-HCT hospitalized patients before and 9 +/- 2 days after initiation of intravenous antibiotic treatment. Fecal microbiota were analyzed by 16s RNA sequencing and urinary 3-IS was analyzed by liquid chromatography-tandem mass spectrometry. Receiver operating characteristic (ROC) analysis was performed to assess the predictive value of 3-IS. Results: At a RAC cutoff of <30%, the binary logarithm of 3-IS (medium 3-IS: <= 2.5; high 3-IS: >2.5) was predictive with an accuracy of 82% (negative predictive value: 87%, positive predictive value 67%). Accuracy was improved by combing antibiotic history with 3-IS levels (accuracy 89%, npv 88%, ppv 92%). Conclusion: In conjunction with patient antibiotic history, 3-IS is a candidate marker to predict RAC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Farowski, Fedja UNSPECIFIED UNSPECIFIED UNSPECIFIED Els, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsakmaklis, Anastasia UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Kahlert, Christian R. UNSPECIFIED orcid.org/0000-0002-0784-3276 UNSPECIFIED Stein-Thoeringer, Christoph K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bobardt, Johanna S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dettmer-Wilde, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Oefner, Peter J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Jorg Janne UNSPECIFIED UNSPECIFIED UNSPECIFIED Vehreschild, Maria J. G. T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-138828
DOI:	10.1080/19490976.2018.1502536
Journal or Publication Title:	Gut Microbes
Volume:	10
Number:	2
Page Range:	S. 133 - 142
Date:	2019
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1949-0984
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIFFICILE-ASSOCIATED DISEASE; CELL TRANSPLANT RECIPIENTS; INFECTION; EPIDEMIOLOGY; ASSOCIATION; MORTALITY; IMPACT Multiple languages Gastroenterology & Hepatology; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13882