Universität zu Köln

Engels, Marianne, Michael, Claire, Dobra, Katalin, Hjerpe, Anders, Fassina, Ambrogio and Firat, Pinar (2019). Management of cytological material, pre-analytical procedures and bio-banking in effusion cytopathology. Cytopathology, 30 (1). S. 31 - 39. HOBOKEN: WILEY. ISSN 1365-2303

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Abstract

Serous effusion fluid is one of the most commonly encountered specimens in routine cytopathology practice. It provides invaluable information about the patient and the clinical status; but to get the most of it, specimen handling and processing must be carried out properly. Cytomorphology is the basis of a successful analysis which should complemented by ancillary tests when needed. A wide spectrum of ancillary techniques - ranging from immunocytochemistry and flow cytometry to different assays of molecular pathology - can be applied to serous effusions. This article describes the acquisition and management of serous effusion fluids, methods for preservation and transportation, different techniques of cytopreparation, application of immunocytochemistry, flow cytometry, and fluorescence in-situ hybridization (FISH), as well as DNA extraction for polymerase chain reaction (PCR) and next generation sequencing (NGS). Principles of bio-banking of effusion samples are also discussed which is getting more important in correlation with the developments in personalized medicine.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Engels, Marianne UNSPECIFIED UNSPECIFIED UNSPECIFIED Michael, Claire UNSPECIFIED UNSPECIFIED UNSPECIFIED Dobra, Katalin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hjerpe, Anders UNSPECIFIED UNSPECIFIED UNSPECIFIED Fassina, Ambrogio UNSPECIFIED UNSPECIFIED UNSPECIFIED Firat, Pinar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-139922
DOI:	10.1111/cyt.12654
Journal or Publication Title:	Cytopathology
Volume:	30
Number:	1
Page Range:	S. 31 - 39
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2303
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SURGICAL PATHOLOGY SPECIMENS; RECEPTOR MUTATION ANALYSIS; LIQUID-BASED CYTOLOGY; CELL-FREE DNA; MALIGNANT MESOTHELIOMA; P16 FISH; DIFFERENTIAL-DIAGNOSIS; ANCILLARY TECHNIQUES; PLEURAL EFFUSIONS; LUNG Multiple languages Cell Biology; Pathology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/13992