Universität zu Köln

Correddu, Danilo ORCID: 0000-0003-4945-8904, Montano Lopez, Jose de Jesus, Angermayr, S. Andreas, Middleditch, Martin J., Payne, Leo S. and Leung, Ivanhoe K. H. (2020). Effect of consecutive rare codons on the recombinant production of human proteins in Escherichia coli. IUBMB Life, 72 (2). S. 266 - 275. HOBOKEN: WILEY. ISSN 1521-6551

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Abstract

In Escherichia coli, the expression of heterologous genes for the production of recombinant proteins can be challenging due to the codon bias of different organisms. The rare codons AGG and AGA are among the rarest in E. coli. In this work, by using the human gene RioK2 as case study, we found that the presence of consecutive AGG-AGA led to a premature stop, which may be caused by an event of -1 frameshift. We found that translational problems caused by consecutive AGG-AGA are sequence dependent, in particular, in sequences that contain multiple rare AGG or AGA codons elsewhere. Translational problems can be alleviated by different strategies, including codon harmonization, codon optimization, or by substituting the consecutive AGG-AGA codons by more frequent arginine codons. Overall, our results furthered our understanding about the relationship between consecutive rare codons and translational problems. Such information will aid the design of DNA sequence for the production of recombinant proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Correddu, Danilo UNSPECIFIED orcid.org/0000-0003-4945-8904 UNSPECIFIED Montano Lopez, Jose de Jesus UNSPECIFIED UNSPECIFIED UNSPECIFIED Angermayr, S. Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Middleditch, Martin J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Payne, Leo S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leung, Ivanhoe K. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-141526
DOI:	10.1002/iub.2162
Journal or Publication Title:	IUBMB Life
Volume:	72
Number:	2
Page Range:	S. 266 - 275
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1521-6551
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRANSFER-RNA; AGA CODONS; TANDEM AGA; EXPRESSION; ARGININE; TRANSLATION Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14152