Universität zu Köln

Ardakani, Ali Abbasian ORCID: 0000-0001-7536-0973, Ghader, Alireza, Asgari, Hamid, Keshavarz, Marzieh, Tazehmahalleh, Fatemeh Ebrahimi, Arae, Mohammad Hosein Majles, Malekzadeh, Malekeh, Ghaznavi, Habib and Shakeri-Zadeh, Ali (2019). The capability of nonlinear optical characteristics as a predictor for cellular uptake of nanoparticles and cell damage. Photodiagnosis Photodyn. Ther., 27. S. 442 - 449. AMSTERDAM: ELSEVIER. ISSN 1873-1597

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Abstract

Current methods for determining the cellular effects of a treatment modality need expensive materials and much time to provide a researcher with results. The aim of this study was to evaluate the potential of nonlinear optical characteristics of cancer cells using Z-scan technique to monitor the level of cellular uptake and cell damage caused by a nanotechnology based treatment modality. Two nanocomplexes were synthesized and characterized. The first one was made of alginate hydrogel co-loaded with cisplatin and gold nanoparticles (AuNPs) named as ACA nanocomplex. The second one, named as AA nanocomplex, was the same as ACA, but without cisplatin and this AA nanocomplex was considered as the control for ACA. Different groups of CT26 mouse colon cancer cell line received various treatments of cisplatin, ACA, and AA nanocomplexes and then the samples were prepared for Z-scan studies. The MTT assay was used to evaluate the cytotoxicity induced by different treatment modalities. Transmission electron microscopy (TEM) and inductively coupled plasma-mass spectrometry (ICP-MS) were used for qualitative and quantitative assessments of the level of AuNPs cellular uptake. The trend of nonlinear optical properties changes for treated cells was in agreement with MTT, TEM and ICP-MS results. Z-scan technique was able to successfully indicate the occurrence of cell damage. It was also capable to determine the intensity of cell damage induced by ACA nanocomplex in comparison to free cisplatin. Furthermore, Z-scan results showed that it was able to discriminate the differences of optical properties of the cells incubated with ACA nanocomplex for various incubation times. Nonlinear optical characteristics of a cell may be considered as a reliable indicator to predict the level of cellular effects induced by a nanotechnology based treatment modality. The protocol suggested in this article does not waste materials, not take much time to provide the results, and it is inexpensive technique.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ardakani, Ali Abbasian UNSPECIFIED orcid.org/0000-0001-7536-0973 UNSPECIFIED Ghader, Alireza UNSPECIFIED UNSPECIFIED UNSPECIFIED Asgari, Hamid UNSPECIFIED UNSPECIFIED UNSPECIFIED Keshavarz, Marzieh UNSPECIFIED UNSPECIFIED UNSPECIFIED Tazehmahalleh, Fatemeh Ebrahimi UNSPECIFIED UNSPECIFIED UNSPECIFIED Arae, Mohammad Hosein Majles UNSPECIFIED UNSPECIFIED UNSPECIFIED Malekzadeh, Malekeh UNSPECIFIED UNSPECIFIED UNSPECIFIED Ghaznavi, Habib UNSPECIFIED UNSPECIFIED UNSPECIFIED Shakeri-Zadeh, Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-142898
DOI:	10.1016/j.pdpdt.2019.07.023
Journal or Publication Title:	Photodiagnosis Photodyn. Ther.
Volume:	27
Page Range:	S. 442 - 449
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-1597
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CISPLATIN; CANCER; CHEMOTHERAPY; DOXORUBICIN; SCATTERING; AUTOPHAGY Multiple languages Oncology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14289