Universität zu Köln

Phillips, C., McNevin, D., Kidd, K. K., Lagace, R., Wootton, S., de la Puente, M., Freire-Aradas, A., Mosquera-Miguel, A., Eduardoff, M., Gross, T., Dagostino, L., Power, D., Olson, S., Hashiyada, M., Oz, C., Parson, W., Schneider, P. M., Lareu, M., V and Daniel, R. (2019). MAPlex - A massively parallel sequencing ancestry analysis multiplex for Asia-Pacific populations. Forensic Sci. Int.-Genet., 42. S. 213 - 227. CLARE: ELSEVIER IRELAND LTD. ISSN 1878-0326

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Abstract

Current forensic ancestry-informative panels are limited in their ability to differentiate populations in the Asia-Pacific region. MAPlex (Multiplex for the Asia-Pacific), a massively parallel sequencing (MPS) assay, was developed to improve differentiation of East Asian, South Asian and Near Oceanian populations found in the extensive cross-continental Asian region that shows complex patterns of admixture at its margins. This study reports the development of MAPlex; the selection of SNPs in combination with microhaplotype markers; assay design considerations for reducing the lengths of microhaplotypes while preserving their ancestry-informativeness; adoption of new population-informative multiple-allele SNPs; compilation of South Asian-informative SNPs suitable for forensic AIMs panels; and the compilation of extensive reference and test population genotypes from online whole-genome-sequence data for MAPlex markers. STRUCTURE genetic clustering software was used to gauge the ability of MAPlex to differentiate a broad set of populations from South and East Asia, the West Pacific regions of Near Oceania, as well as the other globally distributed population groups. Preliminary assessment of MAPlex indicates enhanced South Asian differentiation with increased divergence between West Eurasian, South Asian and East Asian populations, compared to previous forensic SNP panels of comparable scale. In addition, MAPlex shows efficient differentiation of Middle Eastern individuals from Europeans. MAPlex is the first forensic AIM assay to combine binary and multiple-allele SNPs with microhaplotypes, adding the potential to detect and analyze mixed source forensic DNA.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Phillips, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED McNevin, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kidd, K. K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lagace, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wootton, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED de la Puente, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Freire-Aradas, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mosquera-Miguel, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eduardoff, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gross, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dagostino, L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Power, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Olson, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hashiyada, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oz, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Parson, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lareu, M., V UNSPECIFIED UNSPECIFIED UNSPECIFIED Daniel, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143139
DOI:	10.1016/j.fsigen.2019.06.022
Journal or Publication Title:	Forensic Sci. Int.-Genet.
Volume:	42
Page Range:	S. 213 - 227
Date:	2019
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1878-0326
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INFORMATIVE SNP PANEL; MARKERS; MICROHAPLOTYPES; ADMIXTURE; EAST; DIVERSITY; SOUTHWEST; INFERENCE; SELECTION; ASSAY Multiple languages Genetics & Heredity; Medicine, Legal Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14313