Universität zu Köln

Baeza, Luis Lucena, Pfennigwerth, Niels ORCID: 0000-0002-6403-5646 and Hamprecht, Axel ORCID: 0000-0003-1449-5780 (2019). Rapid and Easy Detection of Carbapenemases in Enterobacterales in the Routine Laboratory Using the New GenePOC Carba/Revogene Carba C Assay. J. Clin. Microbiol., 57 (9). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-660X

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Abstract

The novel, real-time PCR-based GenePOC Carba assay on the microfluidic revogene platform (GenePOC, Quebec, Canada; now Meridian Bioscience, Cincinnati, OH, USA) was recently designed for the detection of bla(KPC), bla(NDM), bla(VIM), bla(OXA-48-like), and bla(IMP). The goals of this study were to evaluate the performance of this assay, to assess its suitability for the routine microbiology laboratory, and to compare it to the Xpert Carba-R assay for the detection of carbapenemase-producing Enterobacterales (CPE) strains. The Xpert Carba-R assay (Cepheid) and the GenePOC Carba assay were challenged with a collection of 176 clinical Enterobacte-roles isolates. The collection included 133 CPE strains producing a total of 139 carbapenemases, including VIM (n = 48), OXA-48-like (n = 40), NDM (n = 29), KPC (n = 13), and IMP (n = 9). Six isolates produced two different carbapenemases, and 43 carbapenemase-negative isolates were included as negative controls. The overall sensitivity for carbapenemase detection was 96.4% (95% confidence interval [CI], 91.9% to 98.5%) for the Xpert Carba-R assay and 100% (95% CI, 97.3% to 100%) for the GenePOC assay. The four most common carbapenemases (NDM, KPC, OXA-48-like, and VIM) were detected with a sensitivity of 100% (95% CI, 97.1% to 100%) by the two tests, with all double carbapenemase producers being correctly detected by both assays. The sensitivity of the Xpert Carba-R assay for IMP was 44.4% (95% CI, 18.9% to 73.3%), while that of the GenePOC assay was 100% (95% CI, 70.1% to 100%). The specificity of both assays was 100% (95% CI, 91.8% to 100%). The GenePOC Carba assay showed excellent sensitivity and specificity for the five most common carbapenemases, including IMP variants. Its simplicity and short turnaround time make it suitable for use in the routine microbiology laboratory for CPE detection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Baeza, Luis Lucena UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfennigwerth, Niels UNSPECIFIED orcid.org/0000-0002-6403-5646 UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143160
DOI:	10.1128/JCM.00597-19
Journal or Publication Title:	J. Clin. Microbiol.
Volume:	57
Number:	9
Date:	2019
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-660X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TESTS Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14316