Universität zu Köln

Scherf-Clavel, Oliver ORCID: 0000-0002-1967-9231, Kinzig, Martina, Stoffel, Marc S., Fuhr, Uwe and Soergel, Fritz (2019). Quantification of adefovir and pitavastatin in human plasma and urine by LC-MS/MS: A useful tool for drug-drug interaction studies. J. Chromatogr. B, 1125. AMSTERDAM: ELSEVIER. ISSN 1873-376X

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Abstract

As a tool to be used in transporter-mediated drug-drug interaction studies, a sensitive LC-MS/MS method for the simultaneous quantification of adefovir and pitavastatin in human plasma and adefovir in urine was developed and successfully validated. Plasma samples were processed by protein precipitation using methanol with a subsequent concentrating step. Urine samples were diluted using 0.1% formic acid. Separation was achieved on a Synergy Polar-RP reversed phase column (50 x 4.6 mm, 2.5 mu m) in gradient elution using a mobile phase composed of water and 0.1% formic acid and a mixture of methanol and acetonitrile (50:50, v/v) containing 0.1% formic acid at a flow rate of 1.0 mL/min. The linear range covered concentrations from 0.273 to 52.6 ng/mL for adefovir and from 0.539 to 104.2 ng/mL for pitavastatin in human plasma, respectively. The calibration curve for adefovir in urine ranged from 0.104 to 10.0 mu g/mL. The weighted linear regression (1/conc(2)) implied excellent linearity with correlation coefficients >= 0.999.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Scherf-Clavel, Oliver UNSPECIFIED orcid.org/0000-0002-1967-9231 UNSPECIFIED Kinzig, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoffel, Marc S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuhr, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Soergel, Fritz UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143361
DOI:	10.1016/j.jchromb.2019.121718
Journal or Publication Title:	J. Chromatogr. B
Volume:	1125
Date:	2019
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-376X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PERFORMANCE LIQUID-CHROMATOGRAPHY; COA REDUCTASE INHIBITOR; MASS-SPECTROMETRY; HUMAN SERUM; RAT PLASMA; TRANSPORTER; DIPIVOXIL; VIRUS; EFFICACY; LACTONE Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14336