Universität zu Köln

Lass, Anna ORCID: 0000-0002-5885-9868, Szostakowska, Beata, Kontogeorgos, Ioannis, Korzeniewski, Krzysztof, Karamon, Jacek ORCID: 0000-0002-5830-167X, Sulima, Malgorzata ORCID: 0000-0003-3742-7270 and Karanis, Panagiotis (2019). First detection of Echinococcus multilocularis in environmental water sources in endemic areas using capsule filtration and molecular detection methods. Water Res., 160. S. 466 - 475. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 0043-1354

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Abstract

Water is one of the possible transmission routes for water- and foodborne parasites to humans. Echinococcus multilocularis is a parasite, which causes alveolar echinococcosis (AE). Nevertheless, no environmental studies have been performed as yet to confirm the occurrence of E. multilocularis in water supplies. Accordingly, 105 water samples of 50 L volume were collected from surface waters (lakes, rivers, canals) and wells in the Warmia-Masuria Province (Echinococcus endemic area) and Pomerania Province (Echinococcus non-endemic area), Poland. The water was filtered and subsequently analysed with nested PCR and real-time PCR. E. multilocularis DNA was found in two (1.9%) samples, which originated from two lakes localised in the Warmia-Masuria Province. Sequencing of the positive samples confirmed that the PCR products were fragments of the E. multilocularis mitochondrial 12S rRNA gene. This is the first investigation describing E. multilocularis detection in environmental water samples, using molecular diagnostic tools. The results indicate that water could be considered as a potential source of E. multilocularis infections in humans and animals, in endemic areas. (C) 2019 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lass, Anna UNSPECIFIED orcid.org/0000-0002-5885-9868 UNSPECIFIED Szostakowska, Beata UNSPECIFIED UNSPECIFIED UNSPECIFIED Kontogeorgos, Ioannis UNSPECIFIED UNSPECIFIED UNSPECIFIED Korzeniewski, Krzysztof UNSPECIFIED UNSPECIFIED UNSPECIFIED Karamon, Jacek UNSPECIFIED orcid.org/0000-0002-5830-167X UNSPECIFIED Sulima, Malgorzata UNSPECIFIED orcid.org/0000-0003-3742-7270 UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143714
DOI:	10.1016/j.watres.2019.05.050
Journal or Publication Title:	Water Res.
Volume:	160
Page Range:	S. 466 - 475
Date:	2019
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	0043-1354
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN CYSTIC ECHINOCOCCOSIS; HUMAN ALVEOLAR ECHINOCOCCOSIS; REAL-TIME PCR; RISK-FACTORS; DRINKING-WATER; MASURIA PROVINCE; RED FOXES; EGGS; DNA; CONTAMINATION Multiple languages Engineering, Environmental; Environmental Sciences; Water Resources Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14371