Universität zu Köln

Di Bella, Daniela J., Carcagno, Abel L., Lucia Bartolomeu, M., Belen Pardi, M., Loehr, Heiko, Siegel, Nicole, Hammerschmidt, Matthias, Marin-Burgin, Antonia and Lanuza, Guillermo M. (2019). Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal. Cell Reports, 28 (9). S. 2264 - 2278. CAMBRIDGE: CELL PRESS. ISSN 2211-1247

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Abstract

Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Di Bella, Daniela J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Carcagno, Abel L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lucia Bartolomeu, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Belen Pardi, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Loehr, Heiko UNSPECIFIED UNSPECIFIED UNSPECIFIED Siegel, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Hammerschmidt, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin-Burgin, Antonia UNSPECIFIED UNSPECIFIED UNSPECIFIED Lanuza, Guillermo M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-143888
DOI:	10.1016/j.celrep.2019.07.087
Journal or Publication Title:	Cell Reports
Volume:	28
Number:	9
Page Range:	S. 2264 - 2278
Date:	2019
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2211-1247
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FLUID-CONTACTING NEURONS; TRANSCRIPTION FACTORS; GENE-EXPRESSION; INTERNEURON DIVERSITY; RADIAL GLIA; CELL FATES; STEM-CELLS; MASH1; DIFFERENTIATION; SPECIFICATION Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14388