Universität zu Köln

Sapp, Melanie ORCID: 0000-0002-0817-5425, Tyborski, Nicolas, Linstaedter, Anja, Lopez Sanchez, Aida, Mansfeldt, Tim, Waldhoff, Guido, Bareth, Georg, Bonkowski, Michael and Rose, Laura E. (2019). Site-specific distribution of oak rhizosphere-associated oomycetes revealed by cytochrome c oxidase subunit II metabarcoding. Ecol. Evol., 9 (18). S. 10567 - 10582. HOBOKEN: WILEY. ISSN 2045-7758

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Abstract

The phylum Oomycota comprises important tree pathogens like Phytophthora quercina, involved in central European oak decline, and Phytophthora cinnamomi shown to affect holm oaks among many other hosts. Despite the importance to study the distribution, dispersal and niche partitioning of this phylum, metabarcoding surveys, and studies considering environmental factors that could explain oomycete community patterns are still rare. We investigated oomycetes in the rhizosphere of evergreen oaks in a Spanish oak woodland using metabarcoding based on Illumina sequencing of the taxonomic marker cytochrome c oxidase subunit II (cox2). We developed an approach amplifying a 333 bp long fragment using the forward primer Hud-F (Mycologia, 2000) and a reverse primer found using DegePrime (Applied and Environmental Microbiology, 2014). Factors reflecting topo-edaphic conditions and tree health were linked to oomycete community patterns. The majority of detected OTUs belonged to the Peronosporales. Most taxa were relatives of the Pythiaceae, but relatives of the Peronosporaceae and members of the Saprolegniales were also found. The most abundant OTUs were related to Globisporangium irregulare and P. cinnamomi, both displaying strong site-specific patterns. Oomycete communities were strongly correlated with the environmental factors: altitude, crown foliation, slope and soil skeleton and soil nitrogen. Our findings illustrate the significance of small scale variation in habitat conditions for the distribution of oomycetes and highlight the importance to study oomycete communities in relation to such ecological patterns.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sapp, Melanie UNSPECIFIED orcid.org/0000-0002-0817-5425 UNSPECIFIED Tyborski, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Linstaedter, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Lopez Sanchez, Aida UNSPECIFIED UNSPECIFIED UNSPECIFIED Mansfeldt, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Waldhoff, Guido UNSPECIFIED UNSPECIFIED UNSPECIFIED Bareth, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonkowski, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Rose, Laura E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-144218
DOI:	10.1002/ece3.5577
Journal or Publication Title:	Ecol. Evol.
Volume:	9
Number:	18
Page Range:	S. 10567 - 10582
Date:	2019
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2045-7758
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Geosciences
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHYTOPHTHORA-CINNAMOMI; PHYLOGENETIC-RELATIONSHIPS; ROOT-ROT; FUNGAL COMMUNITIES; 1ST REPORT; DECLINE; SOIL; PYTHIUM; SEQUENCE; FORESTS Multiple languages Ecology; Evolutionary Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/14421